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Q. No.Which of the following is the correct representation of the recognition sequence of EcoR I?
1.
2.
3.
4.
Subtopic: Restriction Enzymes - Main Enzymes |
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Level 1: 80%+
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Which of the following can replicate within bacterial cells independent of the control of chromosomal DNA?
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | Plasmid |
| II: | Phage DNA |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Cloning Vector |
55%
Level 3: 35%-60%
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Consider the given two statements:
| Assertion (A): | In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. |
| Reason (R): | All bacterial cells already have plasmids and will not normally pick up another. |
| 1. | Both (A) and (R) are True and (R) correctly explains the (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain the (A) |
Subtopic: Cloning Vector |
59%
Level 3: 35%-60%
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Consider the given two statements:
1. Statement I is correct; Statement II is incorrect
2. Statement I is incorrect; Statement II is incorrect
3. Statement I is correct; Statement II is correct
4. Statement I is incorrect; Statement II is correct
| Statement I: | There is a need for the recombinant DNA to be expressed in heterologous host. |
| Statement II: | In almost all recombinant technologies, the ultimate aim is to produce a desirable protein. |
1. Statement I is correct; Statement II is incorrect
2. Statement I is incorrect; Statement II is incorrect
3. Statement I is correct; Statement II is correct
4. Statement I is incorrect; Statement II is correct
Subtopic: Process of Biotech |
85%
Level 1: 80%+
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In the name ‘Sma I’, which of the following is missing?
1. Indicative of generic name
2. Indicative of specific name
3. Indicative of strain
4. Order of identification in the bacterium
1. Indicative of generic name
2. Indicative of specific name
3. Indicative of strain
4. Order of identification in the bacterium
Subtopic: General Design of an rDNA experiment |
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Level 1: 80%+
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Consider the given two statements:
| Assertion (A): | In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. |
| Reason (R): | Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Cloning Vector |
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Level 1: 80%+
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A single-stranded nucleic acid fragment, tagged with a radioactive molecule, that interacts with a complementary sequence of a target nucleic acid is called a:
| 1. | Gene gun | 2. | Gene probe |
| 3. | Snip | 4. | Selectable marker |
Subtopic: Molecular Diagnosis |
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Level 1: 80%+
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After being carried about for 30 cycles, PCR can amplify a single segment of DNA to approximately:
| 1. | 1000 copies | 2. | One lakh copies |
| 3. | One million copies | 4. | One billion copies |
Subtopic: Polymerase Chain Reaction: PCR |
86%
Level 1: 80%+
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The tumor inducing (Ti) plasmid of Agrobacterium tumifaciens:
1. is unique as it is single stranded DNA molecule.
2. is not pathogenic to plants.
3. has now been modified into a cloning vector.
4. is frequently used to transform monocots.
1. is unique as it is single stranded DNA molecule.
2. is not pathogenic to plants.
3. has now been modified into a cloning vector.
4. is frequently used to transform monocots.
Subtopic: Cloning Vector |
84%
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In E. coli cloning vector pBR322, restriction site of which of the following restriction enzymes does not lie within a gene for antibiotic resistance?
| 1. | Pvu I | 2. | BamH I |
| 3. | Pst I | 4. | Pvu II |
Subtopic: Selectable Markers: III |
80%
Level 1: 80%+
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