Q. No.The promoter site and the terminator site for transcription are located at:
1.
3' (downstream) end and 5' (upstream) end, respectively of the transcription unit
2.
5' (upstream) end and 3' (downstream) end, respectively of the transcription unit
3.
the 5' (upstream) end
4.
the 3' (downstream) end
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With regard to mature mRNA in eukaryotes:
| 1. | exons and introns do not appear in the mature RNA. |
| 2. | exons appear but introns do not appear in the mature RNA. |
| 3. | introns appear but exons do not appear in the mature RNA. |
| 4. | both exons and introns appear in the mature RNA. |
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Discontinuous synthesis of DNA occurs in one strand, because:
| 1. | DNA molecule being synthesised is very long |
| 2. | DNA-dependent DNA polymerase catalyses polymerisation only in one direction (5’ → 3’) |
| 3. | it is a more efficient process |
| 4. | DNA ligase joins the short stretches of DNA |
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Which of the following statements is correct about the role of regulatory proteins in transcription in prokaryotes?
| 1. | They only increase the expression |
| 2. | They only decrease the expression |
| 3. | They interact with RNA polymerase but do not affect the expression |
| 4. | They can act both as activators and as repressors |
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While analysing the DNA of an organism a total number of 5386 nucleotides were found out of which the proportion of different bases were: Adenine = 29%, Guanine = 17%, Cytosine = 32%, Thymine = 17%. Considering Chargaff’s rule it can be concluded that:
| 1. | it is a double-stranded circular DNA |
| 2. | It is single-stranded DNA |
| 3. | It is a double-stranded linear DNA |
| 4. | No conclusion can be drawn |
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DNA fingerprinting is related to:
| 1. | Molecular analysis of profiles of DNA samples |
| 2. | Analysis of DNA samples using imprinting devices |
| 3. | Techniques used for molecular analysis of different specimens of DNA |
| 4. | Techniques used in the identification of fingerprints of different persons |
A nutritionally wild-type organism that does not require additional biochemicals is:
| 1. | Prototroph | 2. | Auxotroph |
| 3. | Phenotype | 4. | Autotroph |
What is it that forms the basis of DNA Fingerprinting?
| 1. | The relative proportions of purines and pyrimidines in DNA. |
| 2. | The relative difference in the DNA occurrence in blood, skin, and saliva. |
| 3. | The relative amount of DNA in the ridges and grooves of the fingerprints. |
| 4. | Satellite DNA occurring as highly repeated short DNA segments |
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Select the correct option:
|
The direction of RNA synthesis |
The direction of reading of the template DNA strand |
|
|
1. |
5' - 3' |
3' - 5' |
|
2. |
3' - 5' |
5' - 3' |
|
3. |
5' - 3' |
5' - 3' |
|
4. |
3' - 5' |
3' - 5' |
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Which of the following is not required for any of the techniques of DNA fingerprinting available at present?
| 1. | DNA -DNA hybridization | 2. | Polymerase chain reaction |
| 3. | Zinc finger analysis | 4. | Restriction enzymes |
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