Q. No.The correct order of steps in Polymerase Chain Reaction (PCR) is:
1. Extension, Denaturation, Annealing
2. Annealing, Extension, Denaturation
3. Denaturation, Extension, Annealing
4. Denaturation, Annealing, Extension
1. Extension, Denaturation, Annealing
2. Annealing, Extension, Denaturation
3. Denaturation, Extension, Annealing
4. Denaturation, Annealing, Extension
DNA fragments are:
1. Negatively charged
2. Neutral
3. Either positively charged depending on their size
4. Positively charged
2. DNase I
3. RNase
4. Hind II
1. Circular structure
2. Transferable
3. Single-stranded
4. Independent replication
The introduction of t-DNA into plants involves?
1. Infection of the plant by Agrobacterium tumefaciens
2. Altering the pH of the soil, heat-shocking the plants
3. Exposing the plants to Cold for a brief period
4. Allowing the plant roots to stand in water
Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain-
1. Lipase
2. protease
3. Exonuclease
4. Endonuclease
Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
1. Plant cells- cellulase
2. Algae - Methylase
3. Fungi - Chitinase
4. Bacteria - Lysozyme
Which one is a true statement regarding DNA polymerase used in PCR?
| 1. | It is used to ligate introduces DNA in recipient cells |
| 2. | It serves as a selectable marker |
| 3. | It is isolated from a virus |
| 4. | It remains active at high temperatures |
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
Restriction endonucleases are enzymes which:
| 1. | make cuts at specific positions within the DNA molecule |
| 2. | recognize a specific nucleotide sequence for binding of DNA ligase |
| 3. | restrict the action of the enzyme DNA polymerase |
| 4. | remove nucleotides from the ends of the DNA molecule |
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