E.coli is  a commonly used host for gene cloning because

1. It is free from elements that interfere with replication and recombination of DNA

2. It is easy to transform

3. It supports replication of inserted DNA.

4. All of these

Subtopic:  Host & Desired DNA: I | Host & Desired DNA |
 93%
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A gene has been inserted into a vector for the purpose of cloning the sequence only. This vector is called

a.  Suppression vector

b. Expression vector

c. Cloning vector

d. None of these

Subtopic:  Tools: Vectors: I | Tools |
 92%
From NCERT
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Electroporation facilitates insertion of foreign DNA into the cell by

1. Changing the porocity of the membrane

2. Changing the electrical potential of the membrane

3. Lysing the cell wall

4. Active transport

Subtopic:  Tools: Vectors: II | Tools |
 54%
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The names associated with construction of first Recombinant DNA are

a. Arber, Nathans, Smith

b. Annie Chang, Boyer, Berg, Cohen

c. Howard Temin, Brenner, Sharp

d. Tim Hunt, Hartwell, Nurse

Subtopic:  Tools: Enzymes: I | Tools |
 72%
From NCERT
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The restriction enzymes most commonly used in rDNA technology are

1. Type 1 Restriction enzymes

2. Type II Restriction enzymes

3. Type III Restriction enzymes

4. Type IV Restriction enzymes

Subtopic:  Tools: Enzymes: II | Tools |
 78%
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Restriction enzymes do not act on the the DNA of the Host cell in which they are produced because
a. Host DNA is packed into chromosomes
b. Restriction enzymes are ineffective on host DNA
c. Host DNA does not have the restriction site for the Restriction enzymes.
d. Restriction site of host DNA is methylated.

Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II | Tools |
 55%
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A protein is not expressed properly in a diseased tissue. To find out whether the defect is at the level of translation or post translational modifications which techniques would you use ?
a. Southern blotting and South Western blotting
b. Northern blotting and western blotting
c. Western blotting and Eastern blotting
d. Southern and Northern blotting

Subtopic:  Process of Biotech |
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Insertional inactivation of a gene helps in
a. Identification of recombinant clones
b. Identification of deletion mutants
c. Identification of recombinant transformants
d. Elimination of suppression mutants

Subtopic:  Selectable Markers: III | Selectable Markers |
 78%
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Biolistic method makes use of microparticles coated with DNA bombarded at cells to be transformed. These particle are made up of
a. Zinc or tungsten
b. Silicon or gold
c. Tungsten or gold
d. Selenium or Platinum

Subtopic:  Tools: Vectors: II | Tools |
 95%
From NCERT
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Insertional inactivation of the lac Z gene forms-

(1) Blue recombinant colonies

(2) Colourless recombinant colonies

(3) Fluorescent green colonies

(4) There is no relation between the lac Z gene and colour of the colony.

Subtopic:  Selectable Markers: III | Selectable Markers |
 70%
From NCERT
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