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Q. No.The common restriction enzyme EcoRI recognizes the palindromic sequence:
1. GAATTC
2. CCCGGG
3. AAGCTT
4. TGGCCA
Subtopic: Restriction Enzymes - Main Enzymes |
98%
Level 1: 80%+
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What differentiating feature is the basis of separation of fragments of DNA produced by restriction
enzymes in agar gel electrophoresis?
1. charge on the fragments
2. whether they are double or single stranded
3. their size
4. whether linear or circular
enzymes in agar gel electrophoresis?
1. charge on the fragments
2. whether they are double or single stranded
3. their size
4. whether linear or circular
Subtopic: Separation and Isolation of DNA fragments |
93%
Level 1: 80%+
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A plasmid DNA has one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis:
| 1. | only one fragment is seen |
| 2. | two fragments are seen |
| 3. | the cut plasmid DNA will not move and so no fragment is seen |
| 4. | a large number of small sized fragments are seen |
Subtopic: Separation and Isolation of DNA fragments |
Level 3: 35%-60%
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It is usually possible to make bacterial cells more permeable to take up plasmid by treatment with:
1. alkali
2. ultrasound
3. divalent calcium ions
4. trivalent phosphate ions
1. alkali
2. ultrasound
3. divalent calcium ions
4. trivalent phosphate ions
Subtopic: Obtaining Copy of Gene from Donor DNA |
96%
Level 1: 80%+
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The ‘sticky ends’ produced in the fragments of DNA produced by some restriction enzymes are composed of:
| 1. | methyl groups | 2. | telomeres |
| 3. | unpaired bases | 4. | Guanine rich repeats |
Subtopic: Restriction Enzymes - Main Enzymes |
71%
Level 2: 60%+
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Resistance to certain antibiotics in bacterial cells:
I: is a genetic trait
II: is normally carried on the main chromosomal DNA
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
I: is a genetic trait
II: is normally carried on the main chromosomal DNA
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Tools: Vectors: I |
51%
Level 3: 35%-60%
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Bacterial cells add methyl groups to their DNA in order to:
1. clone their DNA
2. activate promoters
3. form polycisronic mRNAs
4. protect its DNA from own restriction enzymes
1. clone their DNA
2. activate promoters
3. form polycisronic mRNAs
4. protect its DNA from own restriction enzymes
Subtopic: Restriction Enzymes - Main Enzymes |
89%
Level 1: 80%+
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What can be the natural function of a restriction enzyme?
| 1. | To remove introns from hnRNA |
| 2. | To cut dsRNA resulting in the formation of miRNA |
| 3. | To cut foreign DNA |
| 4. | To join exons after removal of introns from a transcript |
Subtopic: Introduction & History: II |
85%
Level 1: 80%+
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Restriction enzymes have been isolated from:
I: Bacteria
II: Yeasts
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
I: Bacteria
II: Yeasts
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Restriction Enzymes - Main Enzymes |
74%
Level 2: 60%+
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Maintenance of sterile ambience in chemical engineering processes to enable growth of only the desired microbe/eukaryotic cell in large quantities for the manufacture of biotechnological products like antibiotics, vaccines, enzymes, etc is called as:
| 1. | Genetic engineering | 2. | Bioprocess engineering |
| 3. | Gene technology | 4. | Biopharming |
Subtopic: Introduction & History: II |
85%
Level 1: 80%+
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