Stirred-tank bioreactors have been designed for:

1. purification of product

2. addition of preservatives to the product

3. availability of oxygen throughout the process

4. ensuring anaerobic conditions in the culture vessel

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

(1) Eco RI                       

(2) taq polymerase

(3) polymerase III           

(4) ligase

Which of the following is not a component of downstream processing?

1. Separation

2. Purification

3. Preservation

4. Expression

Which of the following restriction enzymes produces blunt ends?

1. Sal I                           
2. Eco RV
3. Xho                           
4. Hind III

Which of the following is a endonuclease?

(1) Protease

(2) DNase I

(3) RNase restriction

(4) Hind II

The Taq polymerase enzyme is obtained from

1. Thiobacillus ferroxidans

2. Bacillus subtilis

3. Pseudomonas subtilis

4. Thermus aquaticus

The cutting of DNA at specific locations became possible with the discovery of 

(1) restriction enzymes

(2) probes

(3) selectable markets

(4) ligases

The DNA molecule to which the gene of interest is integrated for cloning is called:

Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. Plasmid

4. Cosmid

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by