DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:

(1) Dark red bands

(2) Bright blue bands

(3) Yellow bands

(4) Bright orange bands

Subtopic:  Separation and Isolation of DNA fragments |
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Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

(1) Extension, Denaturation, Annealing

(2) Annealing, Denaturation, Extension

(3) Denaturation, Annealing, Extension

(4) Denaturation, Extension, Annealing

Subtopic:  Polymerase Chain Reaction: PCR |
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

(1) Histones

(2) Polysaccharides

(3) RNA

(4) DNA

Subtopic:  General Design of an rDNA experiment |
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Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain,

1. it will lead to the lysis of host cells.

2. it will be able to produce a novel protein with dual abilities. 

3. it will not be able to confer ampicillin resistance to the host cell. 

4. the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.

Subtopic:  Cloning Vector |
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A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is: 

1. Palindromic Nucleotide sequences

2. Poly(A) tail sequences

3. Degenerate primer sequence

4. Okazaki sequences

Subtopic:  Restriction Enzymes - Main Enzymes |
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During the process of gene amplification using PCR, very high temperature is not maintained in the beginning, then which of the following PCR will be affected first? 

1. Denaturation

2. Ligation

3. Annealing

4. Extension

Subtopic:  Polymerase Chain Reaction: PCR |
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First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognizing a specific sequence of six base pairs is:

1. EcoR1

2. Adenosine deaminase

3. Thermostable DNA polymerase

4. Hind II

Subtopic:  Restriction Enzymes: Historical Background |
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Match the following techniques or instruments with their usage :

(a) Bioreactor               (i) Separation of DNA fragments

(b) Electrophoresis       (ii) Production of large quantities
                                       of products

(c) PCR                       (iii) Detection of pathogen, based
                                       on antigen - antibody reaction

(d) ELISA                    (iv) Amplification of nucleic acids

Select the correct option from following:

1. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)

2. (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii)

3. (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i)

4. (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv)

Subtopic:  Polymerase Chain Reaction: PCR | Bioreactors/Downstream Processing | Separation and Isolation of DNA fragments |
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In a mixture, DNA fragments are separated by-

1. Bioprocess engineering

2. Restriction digestion

3. Electrophoresis

4. Polymerase chain reaction

Subtopic:  Separation and Isolation of DNA fragments |
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Spooling is :-

1. Amplification of DNA

2. Cutting of separated DNA bands from the agarose gel

3. Transfer of separated DNA fragments to synthetic membranes

4. Collection of isolated DNA 

Subtopic:  Separation and Isolation of DNA fragments |
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