The DNA molecule to which the gene of interest is integrated for cloning is called:

1. Transformer 2. Vector
3. Template 4. Carrier 
Subtopic:  Tools: Vectors: I | Tools |
 84%
From NCERT
NEET - 2015
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The cutting of DNA at specific locations became possible with the discovery of?

1. Restriction enzymes

2. Probes

3. Selectable markets

4. ligases

Subtopic:  Tools: Enzymes: II | Tools |
 93%
From NCERT
NEET - 2015
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The introduction of t-DNA into plants involves?

1. Infection of the plant by Agrobacterium tumefaciens

2. Altering the pH of the soil, heat-shocking the plants

3. Exposing the plants to Cold for a brief period

4. Allowing the plant roots to stand in water

Subtopic:  Tools: Vectors: II | Tools |
 91%
From NCERT
NEET - 2015
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Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. Plasmid

4. Cosmid

Subtopic:  Tools: Vectors: I | Tools |
 79%
AIPMT - 2014
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Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

1. Plant cells- cellulose

2. Algae - Methylase

3. Fungi - Chitinase

4. Bacteria - Lysozyme

Subtopic:  Obtaining Copy of Gene from Donor DNA |
 86%
From NCERT
AIPMT - 2013
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:

1. Polymerase chain reaction

2. Electrophoresis

3. Restriction mapping

4. Centrifugation

Subtopic:  Separation and Isolation of DNA fragments |
 92%
From NCERT
AIPMT - 2013
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The colonies of recombinant bacteria appear white because of:

1. Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
2. Insertional inactivation of beta-galactosidase in recombinant bacteria
3. Inactivation of glycosidase enzyme in recombinant bacteria
4. Non-recombinant bacteria containing beta-galactosidase
Subtopic:  Selectable Markers: III | Selection of Recombinant Transformants | Selectable Markers |
 79%
From NCERT
AIPMT - 2013
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During the process of isolation of DNA, chilled ethanol is added to:
1. Precipitate DNA
2. Break open the cell to release DNA
3. Facilitate action of restriction enzymes
4. Remove proteins such as histones
Subtopic:  Separation and Isolation of DNA fragments |
 86%
From NCERT
NEET - 2013
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The figure below is the diagrammatic representation of the E.coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)? 
 

1. Ori-original restriction enzyme

2. Rop-reduced osmotic pressure

3. Hind III, Eco RI-selectable markers

4. AmpR, tetiR-antibiotic resistance genes

Subtopic:  Tools: Vectors: II | Cloning Vector | Tools |
 85%
From NCERT
AIPMT - 2012
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Which one of the following is a case of wrong matching?

1. Somatic hybridization- Fusion of two diverse cells
2. Vector DNA- Site for tRNA synthesis
3. Micropropagation- In vitro production of plants in large numbers
4. Callus - Unorganized mass of cells produced in tissue culture

Subtopic:  Tools: Vectors: I | Tools |
 77%
From NCERT
AIPMT - 2012
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