The DNA molecule to which the gene of interest is integrated for cloning is called

1. Transformer

2. Vector

3. Template

4. Carrier 

Subtopic:  Tools: Vectors: I | Tools |
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The cutting of DNA at specific locations became possible with the discovery of

1. Restriction enzymes

2. probes

3. Selectable markets

4. ligases

Subtopic:  Tools: Enzymes: II | Tools |
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The introduction of t-DNA into plants involves

1. Infection of the plant by Argobacterium tumefaciens

2. Altering the pH of soil, heat-shocking the plants

3. Exposing the plants to Cold for a brief period

4. Allowing the plant roots to stand in water

Subtopic:  Tools: Vectors: II | Tools |
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Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. plasmid

4. cosmid

Subtopic:  Tools: Vectors: I | Tools |
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Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

1. Plant cells- cellulose

2. Algae - Methylase

3. Fungi - Chitinase

4. Bacteria - Lysozyme

Subtopic:  Obtaining Copy of Gene from Donor DNA |
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

1. Polymerase chain reaction

2. Electrophoresis

3. Restriction mapping

4. Centrifugation

Subtopic:  Separation and Isolation of DNA fragments |
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The colonies of recombinant bacteria appear white because of :

1. Insertional inactivation of alpha-galactosidase in non-recombinant bacteria

2. Insertional inactivation of beta-galactosidase in recombinant bacteria

3. Inactivation of glycosidase enzyme in recombinant bacteria

4. Non-recombinant bacteria containing beta-galactosidase

Subtopic:  Selectable Markers: III | Selection of Recombinant Transformants | Selectable Markers |
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The figure below is the diagrammatic representation of the E. coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)? 

1. Ori-original restriction enzyme

2. Rop-reduced osmotic pressure

3. Hind III, Eco RI-selectable markers

4. AmpR, tetiR-antibiotic resistance genes

Subtopic:  Tools: Vectors: II | Cloning Vector | Tools |
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Which one of the following is a case of wrong matching?

1. Somatic hybridization- Fusion of two diverse cells

2. Vector DNA- Site for tRNS synthesis

3. Micropropagation- In vitro production of plants in large numbers

4. Callus - Unorganized mass of cells produced in tissue culture

Subtopic:  Tools: Vectors: I | Tools |
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Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduces DNA in recipient cells

2. It serves as a selectable marker

3. It is isolated from a virus

4. It remains active at high temperature

Subtopic:  Polymerase Chain Reaction: PCR |
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