The correct order of steps in Polymerase Chain Reaction (PCR) is

1. Extension, Denaturation, Annealing
2. Annealing, Extension, Denaturation
3. Denaturation, Extension, Annealing
4. Denaturation, Annealing, Extension

Subtopic:  Polymerase Chain Reaction: PCR |
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The process of separation and purification of expressed protein before marketing is called

(1) upstream processing

(2) downstream processing

(3) bioprocessing

(4) postproduction processing

Subtopic:  Large Scale Production: II | Large Scale Production |
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What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

(1) The larger the fragment size, the farther it moves

(2) The smaller the fragment size, the farther it moves

(3) Positively charged fragments move to farther end

(4) Negatively charged fragments do not move

Subtopic:  Process of Biotech |
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A gene, whose expression helps to identify transformed cells is known as

1. selectable marker                 

2. vector

3. plasmid                                 

4. structural gene

Subtopic:  Selectable Markers: II | Selectable Markers |
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The DNA fragments separated on an agarose gel can be visualised after staining with

(1) bromophenol blue

(2) acetocarmine

(3) aniline blue 

(4) ethidium bromide

Subtopic:  Process of Biotech |
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The DNA fragments separated on an agarose gel can be visualized after staining with:

1. Acetocarmine

2. Aniline blue

3. Ethidium bromide

4. Bromophenol blue

Subtopic:  Separation and Isolation of DNA fragments |
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The process of separation and purification of expressed protein before marketing is called:

1. Downstream processing

2. Bioprocessing

3. Post-product processing

4. Upstream processing

Subtopic:  Bioreactors/Downstream Processing |
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DNA fragments are:

1. Negatively charged

2. Neutral

3. Either positively charged depending on their size

4. Positively charged

Subtopic:  Separation and Isolation of DNA fragments |
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A gene whose expression helps to identify transformed cell is known as:

1. Vector

2. Plasmid

3. Structural gene

4. Selectable marker

Subtopic:  Selectable Markers: I | Selectable Markers: II | Selectable Markers: III | Selectable Markers |
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What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

1. The smaller the fragment size, the farther it moves

2. Positively charged fragment move to farther end

3. Negatively charged fragment do not  move 

4. The larger the fragment size, the farther it moves

Subtopic:  Separation and Isolation of DNA fragments |
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