The correct order of steps in Polymerase Chain Reaction (PCR) is:

1. Extension, Denaturation, Annealing
2. Annealing, Extension, Denaturation
3. Denaturation, Extension, Annealing
4. Denaturation, Annealing, Extension

Subtopic:  Polymerase Chain Reaction: PCR |
 83%
From NCERT
NEET - 2018
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The DNA fragments separated on an agarose gel can be visualized after staining with:

1. Acetocarmine

2. Aniline blue

3. Ethidium bromide

4. Bromophenol blue

Subtopic:  Separation and Isolation of DNA fragments |
 93%
From NCERT
NEET - 2017
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The process of separation and purification of expressed protein before marketing is called:

1. Downstream processing

2. Bioprocessing

3. Post-product processing

4. Upstream processing

Subtopic:  Bioreactors/Downstream Processing |
 89%
From NCERT
NEET - 2017
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DNA fragments are:

1. Negatively charged

2. Neutral

3. Either positively charged depending on their size

4. Positively charged

Subtopic:  Separation and Isolation of DNA fragments |
 93%
From NCERT
NEET - 2017
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A gene whose expression helps to identify transformed cell is known as:

1. Vector 2. Plasmid
3. Structural gene 4. Selectable marker
Subtopic:  Selectable Markers | Tools |
 79%
From NCERT
NEET - 2017
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What is the criterion for the movement of DNA fragments on agarose gel during gel electrophoresis?

1. The smaller the fragment size, the farther it moves

2. Positively charged fragment move to farther end

3. Negatively charged fragments do not move 

4. The larger the fragment size, the farther it moves

Subtopic:  Separation and Isolation of DNA fragments |
 90%
From NCERT
NEET - 2017
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Which of the following is not a component of downstream processing?

1. Separation

2. Purification

3. Preservation

4. Expression

Subtopic:  Large Scale Production: II | Large Scale Production |
 77%
From NCERT
NEET - 2016
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A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?

1. Eco Rl

2. taq polymerase

3. polymerase lll

4. ligase

Subtopic:  Tools: Enzymes: I | Tools |
 90%
From NCERT
NEET - 2016
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Which of the following restriction enzymes produces blunt ends?

1. Sal l

2. Eco RV

3. Xho

4. Hind lll

Subtopic:  Restriction Enzymes - Main Enzymes |
 52%
NEET - 2016
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Which of the following is a restriction endonuclease?
1. Protease
2. DNase I
3. RNase
4. Hind II
Subtopic:  Tools: Enzymes: II | Tools |
 95%
From NCERT
NEET - 2016
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