- 1(current)
Q. No.Consider the following statements:
| I. | Ori is responsible for copy number of the linked DNA. |
| II. | Transformation is the process through which a piece of DNA is introduced into a host bacterium. |
| III. | Bacteriophages have very high copy numbers of their genome within the bacterial cell. |
Which of the above statements are true?
1. I and II only
2. I and III only
3. II and III only
4. I, II and III
Subtopic: Â Tools: Vectors: I |Â Transforming Plant & Animal Cell |Â Tools |
 81%
Level 1: 80%+
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Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
| 1. | (a), (c) and (e) only | 2. | (c), (d) and (e) only |
| 3. | (a), (b) and (c) only | 4. | (a), (c), (d) and (e) only |
Subtopic: Â Cloning Vector |Â Tools |
 87%
Level 1: 80%+
NEET - 2022
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Match the dyes listed in column I with their usage listed in column II and choose the correct option
1. a(iv), b(iii), c(i), d(ii)
2. a(ii), b(iv), c(i), d(iii)
3. a(iv), b(i), c(ii), d(iii)
4. a(iii), b(ii), c(i), d(iv)
| Column I | Column II | ||
| a. | Aniline blue | (i) | Used to stain DNA in agarose gel electrophoresis |
| b. | Bromophenol blue | (ii) | stains chromosomes |
| c. | Ethidium bromide | (iii) | Tracking dye |
| d. | Acetocarmine | (iv) | Used to stain collagen |
1. a(iv), b(iii), c(i), d(ii)
2. a(ii), b(iv), c(i), d(iii)
3. a(iv), b(i), c(ii), d(iii)
4. a(iii), b(ii), c(i), d(iv)
Subtopic: Â Tools |
 57%
Level 3: 35%-60%
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Read the following statements:
(i) Restriction endonucleases are obtained only from prokaryotes
(ii) Sma I creats blunt ends in plasmids
(iii) Blunt ends can be made sticky with the help of enzyme phosphatase
(iv) Size of pBR322 is 43 kb
(v) Shuttle vectors possess two types of ori-sites How many of the above statements are incorrect?
1. Three
2. Two
3. One
4. Four
(i) Restriction endonucleases are obtained only from prokaryotes
(ii) Sma I creats blunt ends in plasmids
(iii) Blunt ends can be made sticky with the help of enzyme phosphatase
(iv) Size of pBR322 is 43 kb
(v) Shuttle vectors possess two types of ori-sites How many of the above statements are incorrect?
1. Three
2. Two
3. One
4. Four
Subtopic: Â Tools: Vectors: I |Â Cloning Vector |Â Tools |
 52%
Level 3: 35%-60%
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