If you have a fungal cell and want to extract DNA, what will be the first enzyme to be used?

A.      Chitinase

B.      Lysozyme

C.      Cellulase

D.     Protease

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If a researcher start his work of DNA extraction from a plant cell, the very first enzyme, he would use will be

A.      Cellulase

B.      Protease

C.      Ribonuclease

D.     Chitinase

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If a researcher has to use a bacterial cell to work with after cell wall breaking which enzyme he needs to use?

A.      Cellulase

B.      Chitinase

C.      Lysozyme

D.     Protease

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If a researcher has to use a bacterial cell to isolate DNA from bacterial cell then to remove other nucleic acid, which enzyme should be used?

A.      DNAse

B.      Ribonuclease

C.      Protease

D.     Lysozyme

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The precipitation of DNA out of the solution is done by adding

A.      Organic Acid

B.      HCl

C.      Ethanol

D.     Ketone

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The end result of precipitation of DNA in suspension is seen

A.      In the form of dark solution

B.      In the form of effervescence

C.      In the form of fine threads

D.     In the form of bubbles

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The precipitated DNA can be removed from the suspension

A.      By Filtration

B.      By giving a heat shock

C.      By spooling

D.     By gel electrophoresis

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For the synthesis of 4 copies of DNA, how many sets of primers are needed in PCR

A.      2 sets

B.      8 sets

C.      4 sets

D.     Random

Concept Questions :-

Large Scale Production: I
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The synthesis of DNA in PCR will be in

A.      5’-3’

B.      3’-5’

C.      It won't be depending on direction

D.     For one strand 5’-3’ in another opposite to it

Concept Questions :-

Large Scale Production: I
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The primers designed according to (Answer according to very basic PCR)

A.      Terminal ends of vector

B.      Terminal ends of gene of interest

C.      Random primers

D.     According to direction of PCR

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