Given below is a sample of portion of DNA strand giving the base sequence on the opposite strands. What is so, special shown in it?

5'__ GAATTC_ 3'

3' __ CTTAAG__5'

(a) Deletion mutation

(b) Start codon at the 5' end

(c) Palindromic sequence of base pairs

(d) Replication completed

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There is a restriction endonuclease called Eco RI. What does 'co' part in it stand for?

(a) Coelom                         (b) Coenzyme

(c) coli                               (d) Colon

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Agarose extracted from sea weeds finds use in

(a) tissue culture

(b) PCR

(c) gel electrophoresis

(d) spectrophotometry

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Continuous addition of sugars in ‘fed batch’ fermentation is done to

(a) obtain antibiotics

(b) purify enzymes

(c) degrade sewage

(d) produce methane

Concept Questions :-

Large Scale Production: II
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Which one of the following is used as vector for cloning genes into higher organisms?

(a) Baculovirus

(b) Salmonella typhimurium

(c) Rhizopus nigricans

(d) Retrovirus

Concept Questions :-

Tools: Vectors:II
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Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

(a) 5'-CGTTCG-3'

     3'-ATGGTA--5'

(b) 5'-GATATG-3'

     3'-CTACTA-5'

(c) 5'-GAATTC-3'

     3'-CTTAAG-5'

(d) 5'-CACGTA-3'

     3'-CTCAGT-5'

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DNA or RNA segment tagged with a radioactive molecule is called

(a) vector

(b) probe

(c) clone

(d) plasmid

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Restriction endonucleases are enzymes which
(a) make cuts at specific positions within the DNA molecule
(b) recognize a specific nucleotide sequence for binding of DNA ligase
(c) restrict the action of the enzyme DNA polymerase
(d) remove nucleotides from the ends of the DNA molecule

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Which one of the following is commonly used in transfer of foreign DNA into crop plants?

(a) Trichoderma harzianum

(b) Meloidogyne incognita

(c) Agrobacterium tumefaciens

(d) Penicillium expansum

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Polyethylene glycol method is used for

(a) gene transfer without a vector

(b) biodiesel production

(c) seedless fruit production

(d) energy production from sewage

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