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Q. No.Consider the given two statements:
Assertion (A):
A piece of DNA, if needed to be propagated during recombinant DNA procedures, requires a vector.
Reason (R):
The vectors provide the origin of replication.
1.
Both (A) and (R) are True but (R) does not correctly explain (A).
2.
(A) is False but (R) is True.
3.
(A) is True but (R) is False.
4.
Both (A) and (R) are True and (R) correctly explains (A).
Subtopic: Tools |
74%
Level 2: 60%+
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Name the British biochemist who received the Nobel Prize in Chemistry twice – once for determining the amino acid sequence of insulin and then for developing first-ever DNA sequencing technique:
1. Karl Barry Sharpless
2. John Bardeen
3. Frederick Sanger
4. Walter Gilbert
1. Karl Barry Sharpless
2. John Bardeen
3. Frederick Sanger
4. Walter Gilbert
Subtopic: Human Genome Project |
87%
Level 1: 80%+
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Techniques to alter the chemistry of genetic material (DNA and RNA), to introduce these into host organisms and thus change the phenotype of the host organism constitute:
1. Biotechnology
2. Genetic engineering
3. Bioprocess engineering
4. Genomics
1. Biotechnology
2. Genetic engineering
3. Bioprocess engineering
4. Genomics
Subtopic: Introduction & History |
84%
Level 1: 80%+
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A piece of DNA, which is somehow transferred into an alien organism, most likely:
| 1. | would not be able to multiply itself in the progeny cells of the organism. |
| 2. | would be transcribed but the transcript will not be translated. |
| 3. | would express as different proteins from the organism it was derived from. |
| 4. | would be invariably destroyed by the cytosolic endonucleases in eukaryotic cells. |
Subtopic: Introduction & History: II |
62%
Level 2: 60%+
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Which of the following is the correct representation of the recognition sequence of EcoR I?
| 1. |  |
| 2. |  |
| 3. |  |
| 4. |  |
Subtopic: Restriction Enzymes - Main Enzymes |
81%
Level 1: 80%+
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Which of the following can replicate within bacterial cells independent of the control of chromosomal DNA?
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | Plasmid |
| II: | Phage DNA |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Cloning Vector |
55%
Level 3: 35%-60%
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Consider the given two statements:
| Assertion (A): | In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. |
| Reason (R): | All bacterial cells already have plasmids and will not normally pick up another. |
| 1. | Both (A) and (R) are True and (R) correctly explains the (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain the (A) |
Subtopic: Cloning Vector |
59%
Level 3: 35%-60%
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Consider the given two statements:
1. Statement I is correct; Statement II is incorrect
2. Statement I is incorrect; Statement II is incorrect
3. Statement I is correct; Statement II is correct
4. Statement I is incorrect; Statement II is correct
| Statement I: | There is a need for the recombinant DNA to be expressed in heterologous host. |
| Statement II: | In almost all recombinant technologies, the ultimate aim is to produce a desirable protein. |
1. Statement I is correct; Statement II is incorrect
2. Statement I is incorrect; Statement II is incorrect
3. Statement I is correct; Statement II is correct
4. Statement I is incorrect; Statement II is correct
Subtopic: Process of Biotech |
85%
Level 1: 80%+
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In the name ‘Sma I’, which of the following is missing?
1. Indicative of generic name
2. Indicative of specific name
3. Indicative of strain
4. Order of identification in the bacterium
1. Indicative of generic name
2. Indicative of specific name
3. Indicative of strain
4. Order of identification in the bacterium
Subtopic: General Design of an rDNA experiment |
82%
Level 1: 80%+
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Consider the given two statements:
| Assertion (A): | In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. |
| Reason (R): | Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Cloning Vector |
91%
Level 1: 80%+
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