| 1. | would not be able to multiply itself in the progeny cells of the organism. | 
| 2. | would be transcribed but the transcript will not be translated. | 
| 3. | would express as different proteins from the organism it was derived from. | 
| 4. | would be invariably destroyed by the cytosolic endonucleases in eukaryotic cells. | 
| 1. | |
| 2. | |
| 3. | |
| 4. | 
| I: | Plasmid | 
| II: | Phage DNA | 
| Assertion (A): | In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. | 
| Reason (R): | All bacterial cells already have plasmids and will not normally pick up another. | 
| 1. | Both (A) and (R) are True and (R) correctly explains the (A). | 
| 2. | (A) is True; (R) is False | 
| 3. | (A) is False; (R) is False | 
| 4. | Both (A) and (R) are True but (R) does not correctly explain the (A) | 
| Statement I: | There is a need for the recombinant DNA to be expressed in heterologous host. | 
| Statement II: | In almost all recombinant technologies, the ultimate aim is to produce a desirable protein. | 
| Assertion (A): | In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. | 
| Reason (R): | Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. | 
| 1. | Both (A) and (R) are True and (R) correctly explains (A). | 
| 2. | (A) is True; (R) is False | 
| 3. | (A) is False; (R) is False | 
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). | 
| 1. | Gene gun | 2. | Gene probe | 
| 3. | Snip | 4. | Selectable marker | 
| 1. | 1000 copies | 2. | One lakh copies | 
| 3. | One million copies | 4. | One billion copies |