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Q. No.Primers used in PCR are:
1.
a complementary DNA sequence synthesised on the template of hnRNA
2.
artificially synthesised or sequences used to induce replication of DNA
3.
a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome
4.
small chemically synthesised oligonucletides that are complementary to the regions of DNA
Subtopic: Polymerase Chain Reaction: PCR |
73%
Level 2: 60%+
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Regarding stirred-tank reactor:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | It is usually spherical in shape. |
| II: | The stirrer facilitates even mixing and oxygen availability throughout the reactor. |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Bioreactors/Downstream Processing |
65%
Level 2: 60%+
Hints
Identify the correct statement:
| 1. | DNA is the genetic material of all organisms without exception. |
| 2. | As it is an RNA world, the genetic material is ds RNA in majority of the organisms. |
| 3. | Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules. |
| 4. | Proteins such as histones can be removed from the cell lysate with the help of ribonuclease. |
Subtopic: Process of Biotech |
78%
Level 2: 60%+
Hints
In a continuous culture system:
| I: | The used medium is drained out from one side while the fresh medium is added from the other. |
| II: | The cells are maintained in their physiologically most active log/exponential phase. |
| III: | A larger biomass is produced leading to higher yields of desired proteins. |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II and III are correct |
Subtopic: Large Scale Production |
88%
Level 1: 80%+
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Consider the given two statements:
| Assertion (A): | A piece of DNA, if needed to be propagated during recombinant DNA procedures, requires a vector. |
| Reason (R): | The vectors provide the origin of replication. |
| 1. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 2. | (A) is False but (R) is True. |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are True and (R) correctly explains (A). |
Subtopic: Tools |
74%
Level 2: 60%+
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To isolate DNA from a cell, the cell wall needs to be broken. Match the enzymes in Column I with the type of cell in Column II and select the correct match from the codes given:
Codes:
| Column I | Column II | ||
| A. | Lysozyme | P. | Fungal cell |
| B. | Cellulase | Q. | Plant cell |
| C. | Chitinase | R. | Bacterial cell |
| A | B | C | |
| 1. | P | Q | R |
| 2. | P | R | Q |
| 3. | R | P | Q |
| 4. | R | Q | P |
Subtopic: Host & Desired DNA |
92%
Level 1: 80%+
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Name the British biochemist who received the Nobel Prize in Chemistry twice – once for determining the amino acid sequence of insulin and then for developing first-ever DNA sequencing technique:
1. Karl Barry Sharpless
2. John Bardeen
3. Frederick Sanger
4. Walter Gilbert
1. Karl Barry Sharpless
2. John Bardeen
3. Frederick Sanger
4. Walter Gilbert
Subtopic: Human Genome Project |
87%
Level 1: 80%+
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Techniques to alter the chemistry of genetic material (DNA and RNA), to introduce these into host organisms and thus change the phenotype of the host organism constitute:
1. Biotechnology
2. Genetic engineering
3. Bioprocess engineering
4. Genomics
1. Biotechnology
2. Genetic engineering
3. Bioprocess engineering
4. Genomics
Subtopic: Introduction & History |
84%
Level 1: 80%+
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A piece of DNA, which is somehow transferred into an alien organism, most likely:
| 1. | would not be able to multiply itself in the progeny cells of the organism. |
| 2. | would be transcribed but the transcript will not be translated. |
| 3. | would express as different proteins from the organism it was derived from. |
| 4. | would be invariably destroyed by the cytosolic endonucleases in eukaryotic cells. |
Subtopic: Introduction & History: II |
62%
Level 2: 60%+
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Which of the following is the correct representation of the recognition sequence of EcoR I?
| 1. |  |
| 2. |  |
| 3. |  |
| 4. |  |
Subtopic: Restriction Enzymes - Main Enzymes |
81%
Level 1: 80%+
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