Q. No.The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as:
1.
Transduction
2.
Conjugation
3.
Transformation
4.
Translation
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Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
| 1. | DNA can be seen in visible light |
| 2. | DNA can be seen without staining in visible light |
| 3. | Ethidium bromide stained DNA can be seen in visible light |
| 4. | Ethidium bromide stained DNA can be seen under exposure to UV light |
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of the phosphodiester bonds in DNA by the enzyme |
| 2. | Cutting of DNA at a specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Which of the following is not required in the preparation of a recombinant DNA molecule?
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA fragments | 4. | E.coli |
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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
| 1. | Charge only | 2. | Size only |
| 3. | Charge-to-size ratio | 4. | All of the above |
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The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
| 1. | Origin of replication (ori) |
| 2. | Presence of a selectable marker |
| 3. | Presence of sites for restriction endonuclease |
| 4. | Its size |
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While isolating DNA from bacteria, which of the following enzymes is not required?
| 1. | Lysozyme | 2. | Ribonuclease |
| 3. | Deoxyribonuclease | 4. | Protease |
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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
| 1. | Easy availability of DNA template |
| 2. | Availability of synthetic primers |
| 3. | Availability of cheap deoxyribonucleotides |
| 4. | Availability of 'Thermostable' DNA polymerase |
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An antibiotic resistance gene in a vector usually helps in the selection of:
1. Competent bacterial cells
2. Transformed bacterial cells
3. Recombinant bacterial cells
4. None of the above
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The significance of the 'heat shock' method in bacterial transformation is to facilitate:
| 1. | Binding of DNA to the cell wall |
| 2. | Uptake of DNA through membrane transport proteins |
| 3. | Uptake of DNA through transient pores in the bacterial cell wall |
| 4. | Expression of antibiotic resistance gene |
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