Q. No.Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
1.
DNA can be seen in visible light
2.
DNA can be seen without staining in visible light
3.
Ethidium bromide stained DNA can be seen in visible light
4.
Ethidium bromide stained DNA can be seen under exposure to UV light
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of the phosphodiester bonds in DNA by the enzyme |
| 2. | Cutting of DNA at a specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Which of the following is not required in the preparation of a recombinant DNA molecule?
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA fragments | 4. | E.coli |
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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
| 1. | Charge only | 2. | Size only |
| 3. | Charge-to-size ratio | 4. | All of the above |
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The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
| 1. | Origin of replication (ori) |
| 2. | Presence of a selectable marker |
| 3. | Presence of sites for restriction endonuclease |
| 4. | Its size |
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While isolating DNA from bacteria, which of the following enzymes is not required?
| 1. | Lysozyme | 2. | Ribonuclease |
| 3. | Deoxyribonuclease | 4. | Protease |
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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
| 1. | Easy availability of DNA template |
| 2. | Availability of synthetic primers |
| 3. | Availability of cheap deoxyribonucleotides |
| 4. | Availability of 'Thermostable' DNA polymerase |
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An antibiotic resistance gene in a vector usually helps in the selection of:
1. Competent bacterial cells
2. Transformed bacterial cells
3. Recombinant bacterial cells
4. None of the above
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The significance of the 'heat shock' method in bacterial transformation is to facilitate:
| 1. | Binding of DNA to the cell wall |
| 2. | Uptake of DNA through membrane transport proteins |
| 3. | Uptake of DNA through transient pores in the bacterial cell wall |
| 4. | Expression of antibiotic resistance gene |
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The role of DNA ligase in the construction of a recombinant DNA molecule is:
| 1. | Formation of a phosphodiester bond between two DNA fragments |
| 2. | Formation of hydrogen bonds between sticky ends of DNA fragments |
| 3. | Ligation of all purine and pyrimidine bases |
| 4. | None of the above |
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