Q. No.An antibiotic resistance gene in a vector usually helps in the selection of:
1. Competent bacterial cells
2. Transformed bacterial cells
3. Recombinant bacterial cells
4. None of the above
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The significance of the 'heat shock' method in bacterial transformation is to facilitate:
| 1. | Binding of DNA to the cell wall |
| 2. | Uptake of DNA through membrane transport proteins |
| 3. | Uptake of DNA through transient pores in the bacterial cell wall |
| 4. | Expression of antibiotic resistance gene |
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The role of DNA ligase in the construction of a recombinant DNA molecule is:
| 1. | Formation of a phosphodiester bond between two DNA fragments |
| 2. | Formation of hydrogen bonds between sticky ends of DNA fragments |
| 3. | Ligation of all purine and pyrimidine bases |
| 4. | None of the above |
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Which of the following bacteria is not a source of restriction endonuclease?
1. Haemophilusinfluenzae
2. Escherichia coli
3. Entamoeba coli
4. Bacillus amyloliquefaciens
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
1. Denaturation of template DNA
2. Annealing of primers to template DNA
3. Extension of primer end on the template DNA
4. All of the above
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A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be:
| 1. | Human genes may have intron which bacteria cannot process |
| 2. | Amino acid codons for humans and bacteria are different |
| 3. | Human protein is formed but degraded by bacteria |
| 4. | All of the above |
Which of the following should be chosen for the best yield if one were to produce a recombinant protein in large amounts?
| 1. | Laboratory flask of the largest capacity |
| 2. | A stirred-tank bioreactor without in-lets and out-lets |
| 3. | A continuous culture system |
| 4. | Any of the above |
Who among the following was awarded the Nobel Prize for the development of the PCR technique?
| 1. | Herbert Boyer | 2. | Hargovind Khurana |
| 3. | Kary Mullis | 4. | Arthur Kornberg |
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Which of the following statements does not hold true for restriction enzyme?
| 1. | It recognises a palindromic nucleotide sequence |
| 2. | It is an endonuclease |
| 3. | It is isolated from viruses |
| 4. | It can produce the same kind of sticky ends in different DNA molecules |
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Ti-plasmid is used for making transgenic plants. It is obtained from
1. Azotobacter
2. Agrobacterium
3. Rhizobium in leguminous root
4. Yeast
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