Q. No.The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
1.
Origin of replication (ori)
2.
Presence of a selectable marker
3.
Presence of sites for restriction endonuclease
4.
Its size
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While isolating DNA from bacteria, which of the following enzymes is not required?
| 1. | Lysozyme | 2. | Ribonuclease |
| 3. | Deoxyribonuclease | 4. | Protease |
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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
| 1. | Easy availability of DNA template |
| 2. | Availability of synthetic primers |
| 3. | Availability of cheap deoxyribonucleotides |
| 4. | Availability of 'Thermostable' DNA polymerase |
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An antibiotic resistance gene in a vector usually helps in the selection of:
1. Competent bacterial cells
2. Transformed bacterial cells
3. Recombinant bacterial cells
4. None of the above
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The significance of the 'heat shock' method in bacterial transformation is to facilitate:
| 1. | Binding of DNA to the cell wall |
| 2. | Uptake of DNA through membrane transport proteins |
| 3. | Uptake of DNA through transient pores in the bacterial cell wall |
| 4. | Expression of antibiotic resistance gene |
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The role of DNA ligase in the construction of a recombinant DNA molecule is:
| 1. | Formation of a phosphodiester bond between two DNA fragments |
| 2. | Formation of hydrogen bonds between sticky ends of DNA fragments |
| 3. | Ligation of all purine and pyrimidine bases |
| 4. | None of the above |
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Which of the following bacteria is not a source of restriction endonuclease?
1. Haemophilusinfluenzae
2. Escherichia coli
3. Entamoeba coli
4. Bacillus amyloliquefaciens
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
1. Denaturation of template DNA
2. Annealing of primers to template DNA
3. Extension of primer end on the template DNA
4. All of the above
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A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be:
| 1. | Human genes may have intron which bacteria cannot process |
| 2. | Amino acid codons for humans and bacteria are different |
| 3. | Human protein is formed but degraded by bacteria |
| 4. | All of the above |
Which of the following should be chosen for the best yield if one were to produce a recombinant protein in large amounts?
| 1. | Laboratory flask of the largest capacity |
| 2. | A stirred-tank bioreactor without in-lets and out-lets |
| 3. | A continuous culture system |
| 4. | Any of the above |
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