Q. No.Which of the following is normally not used as a method of introduction of foreign DNA into an animal cell?
1.
Microinjection
2.
Liposomes
3.
Biolistics
4.
Transfection
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The rising of dough is due to:
1. Multiplication of yeast
2. Production of CO2
3. Emulsification
4. Hydrolysis of wheat flour starch into sugars
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Which of the following enzymes catalyze the removal of nucleotides from the ends of DNA?
| 1. | endonuclease | 2. | exonuclease |
| 3. | DNA ligase | 4. | Hind – II |
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The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as:
| 1. | Transduction | 2. | Conjugation |
| 3. | Transformation | 4. | Translation |
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Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
| 1. | DNA can be seen in visible light |
| 2. | DNA can be seen without staining in visible light |
| 3. | Ethidium bromide stained DNA can be seen in visible light |
| 4. | Ethidium bromide stained DNA can be seen under exposure to UV light |
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of the phosphodiester bonds in DNA by the enzyme |
| 2. | Cutting of DNA at a specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Which of the following is not required in the preparation of a recombinant DNA molecule?
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA fragments | 4. | E.coli |
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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
| 1. | Charge only | 2. | Size only |
| 3. | Charge-to-size ratio | 4. | All of the above |
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The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
| 1. | Origin of replication (ori) |
| 2. | Presence of a selectable marker |
| 3. | Presence of sites for restriction endonuclease |
| 4. | Its size |
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While isolating DNA from bacteria, which of the following enzymes is not required?
| 1. | Lysozyme | 2. | Ribonuclease |
| 3. | Deoxyribonuclease | 4. | Protease |
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