Q. No.Identify the incorrectly matched pair:
restriction enzyme recognition site
1. EcoRI 5’GAATTC3’
3’CTTAAG5’
2. HindII 5’GTPyPuAC3’
3’CAPuPyTG3’
3. SmaI 5’GGATTC3’
3’CTTAGG5’
4. HindIII 5’AAGCTT3’
3’TTCGAA5’
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Which of the following restriction enzymes would not produce “sticky ends”?
| 1. | EcoRI | 2. | HindII |
| 3. | BamHI | 4. | HindIII |
If the required gene has been isolated by shotgun approach, care must be taken to:
| 1. | cut the plasmid with a different restriction enzyme than that used to obtain the donor gene |
| 2. | cut the plasmid with the same restriction enzyme that was used to obtain the donor gene |
| 3. | avoid cutting the plasmid |
| 4. | cut the plasmid with two different restriction enzymes |
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The chronological steps of a polymerase chain reaction are:
| 1. | Denaturation, Annealing of primers, Primer extension |
| 2. | Annealing of primers, Denaturation, Primer extension |
| 3. | Annealing of primers, Primer extension, Denaturation |
| 4. | Denaturation, Primer extension, Annealing of primers |
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Taq polymerase is used in the polymerase chain reaction because:
| 1. | It replicates DNA faster than other enzymes |
| 2. | It is the only enzyme that can replicate DNA invitro |
| 3. | It does not require an RNA primer to function |
| 4. | It is thermostable |
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A major advantage of using YAC as a cloning vector over the plasmids is that:
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites
Which of the following is normally not used as a method of introduction of foreign DNA into an animal cell?
| 1. | Microinjection | 2. | Liposomes |
| 3. | Biolistics | 4. | Transfection |
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The rising of dough is due to:
1. Multiplication of yeast
2. Production of CO2
3. Emulsification
4. Hydrolysis of wheat flour starch into sugars
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Which of the following enzymes catalyze the removal of nucleotides from the ends of DNA?
| 1. | endonuclease | 2. | exonuclease |
| 3. | DNA ligase | 4. | Hind – II |
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The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as:
| 1. | Transduction | 2. | Conjugation |
| 3. | Transformation | 4. | Translation |
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