Q. No.The chronological steps of a polymerase chain reaction are:
1.
Denaturation, Annealing of primers, Primer extension
2.
Annealing of primers, Denaturation, Primer extension
3.
Annealing of primers, Primer extension, Denaturation
4.
Denaturation, Primer extension, Annealing of primers
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Taq polymerase is used in the polymerase chain reaction because:
| 1. | It replicates DNA faster than other enzymes |
| 2. | It is the only enzyme that can replicate DNA invitro |
| 3. | It does not require an RNA primer to function |
| 4. | It is thermostable |
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A major advantage of using YAC as a cloning vector over the plasmids is that:
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites
Which of the following is normally not used as a method of introduction of foreign DNA into an animal cell?
| 1. | Microinjection | 2. | Liposomes |
| 3. | Biolistics | 4. | Transfection |
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The rising of dough is due to:
1. Multiplication of yeast
2. Production of CO2
3. Emulsification
4. Hydrolysis of wheat flour starch into sugars
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Which of the following enzymes catalyze the removal of nucleotides from the ends of DNA?
| 1. | endonuclease | 2. | exonuclease |
| 3. | DNA ligase | 4. | Hind – II |
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The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as:
| 1. | Transduction | 2. | Conjugation |
| 3. | Transformation | 4. | Translation |
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Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
| 1. | DNA can be seen in visible light |
| 2. | DNA can be seen without staining in visible light |
| 3. | Ethidium bromide stained DNA can be seen in visible light |
| 4. | Ethidium bromide stained DNA can be seen under exposure to UV light |
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of the phosphodiester bonds in DNA by the enzyme |
| 2. | Cutting of DNA at a specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Which of the following is not required in the preparation of a recombinant DNA molecule?
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA fragments | 4. | E.coli |
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