| A | B | C | D | |
| 1 | Transition State | Potential energy | Activation energy without enzyme | Activation energy with enzyme |
| 2 | Kinetic energy | Potential energy | Activation energy without enzyme | Activation energy with enzyme |
| 3 | Potential energy | Transition state | Activation energy without enzyme | Activation energy with enzyme |
| 4 | Potential energy | Transition state | Activation energy without enzyme | Activation energy with enzyme |
| a. | The substrate binds to the active site of the enzyme. |
| b. | Enzymes isolated form thermophilic organisms get denatured at 50o C. |
| c. | The active site of enzyme breaks the chemical bonds of the product. |
| d. | Prosthetic groups are tightly bound to the apoenzyme. |
| 1. | (a) & (c) | 2. | (c) & (d) |
| 3. | (b) & (c) | 4. | (a) & (d) |