Select Question Set:
Q. No.The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
1.
EcoRI
2.
HindII
3.
SmaI
4.
BamHI
Subtopic: Tools: Enzymes: I | Tools: Enzymes: II |
92%
Level 1: 80%+
Hints
Gene cloning occurs:
| 1. | when a phage transfers bacterial DNA from one bacterium to another. |
| 2. | when a bacterium takes up DNA from the surrounding fluid. |
| 3. | when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. |
| 4. | when DNA is produced from an RNA template. |
Subtopic: Process of Biotech |
93%
Level 1: 80%+
Hints
Which of the following is not required in the preparation of a recombinant DNA molecule?
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA fragments | 4. | E.coli |
Subtopic: Tools | General Design of an rDNA experiment |
75%
Level 2: 60%+
Hints
‘Downstream processing’ does not include:
| 1. | Product biosynthesis | 2. | Product isolation |
| 3. | Product purification | 4. | Product polishing |
Subtopic: Bioreactors/Downstream Processing |
59%
Level 3: 35%-60%
Hints
The chronological steps of a polymerase chain reaction are:
| 1. | Denaturation, Annealing of primers, Primer extension |
| 2. | Annealing of primers, Denaturation, Primer extension |
| 3. | Annealing of primers, Primer extension, Denaturation |
| 4. | Denaturation, Primer extension, Annealing of primers |
Subtopic: Polymerase Chain Reaction: PCR |
90%
Level 1: 80%+
Hints
The advantage of using DNA polymerases from thermophilic organisms in PCR is that:
| 1. | the DNA polymerases of these bacteria are much faster than those from other organisms |
| 2. | the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands |
| 3. | the DNA polymerases of these bacteria never make mistakes while replicating DNA |
| 4. | all of the above |
Subtopic: Process of Biotech |
74%
Level 2: 60%+
Hints
To isolate DNA from a cell, the cell wall needs to be broken. Match the enzymes in Column I with the type of cell in Column II and select the correct match from the codes given:
Codes:
| Column I | Column II | ||
| A. | Lysozyme | P. | Fungal cell |
| B. | Cellulase | Q. | Plant cell |
| C. | Chitinase | R. | Bacterial cell |
| A | B | C | |
| 1. | P | Q | R |
| 2. | P | R | Q |
| 3. | R | P | Q |
| 4. | R | Q | P |
Subtopic: Host & Desired DNA |
92%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
In a continuous culture system:
| I: | The used medium is drained out from one side while the fresh medium is added from the other. |
| II: | The cells are maintained in their physiologically most active log/exponential phase. |
| III: | A larger biomass is produced leading to higher yields of desired proteins. |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II and III are correct |
Subtopic: Large Scale Production |
88%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
Identify the correct statement:
| 1. | DNA is the genetic material of all organisms without exception. |
| 2. | As it is an RNA world, the genetic material is ds RNA in majority of the organisms. |
| 3. | Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules. |
| 4. | Proteins such as histones can be removed from the cell lysate with the help of ribonuclease. |
Subtopic: Process of Biotech |
78%
Level 2: 60%+
Hints
Primers used in PCR are:
| 1. | a complementary DNA sequence synthesised on the template of hnRNA |
| 2. | artificially synthesised or sequences used to induce replication of DNA |
| 3. | a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome |
| 4. | small chemically synthesised oligonucletides that are complementary to the regions of DNA |
Subtopic: Polymerase Chain Reaction: PCR |
73%
Level 2: 60%+
Hints
Select Question Set:
© 2025 GoodEd Technologies Pvt. Ltd.