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Q. No.

Which of the following is not required in the preparation of a recombinant DNA molecule?

1. Restriction endonuclease 2. DNA ligase
3. DNA fragments 4. E.coli

Subtopic:  Tools | General Design of an rDNA experiment |
 75%
Level 2: 60%+
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The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:

1. EcoRI 2. HindII
3. SmaI 4. BamHI
Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II |
 92%
Level 1: 80%+
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Gene cloning occurs:

1. when a phage transfers bacterial DNA from one bacterium to another.
2. when a bacterium takes up DNA from the surrounding fluid.
3. when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid.
4. when DNA is produced from an RNA template.

Subtopic:  Process of Biotech |
 93%
Level 1: 80%+
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‘Downstream processing’ does not include:

1. Product biosynthesis 2. Product isolation
3. Product purification 4. Product polishing

Subtopic:  Bioreactors/Downstream Processing |
 59%
Level 3: 35%-60%
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The chronological steps of a polymerase chain reaction are:

1. Denaturation, Annealing of primers, Primer extension
2. Annealing of primers, Denaturation, Primer extension
3. Annealing of primers, Primer extension, Denaturation
4. Denaturation, Primer extension, Annealing of primers
Subtopic:  Polymerase Chain Reaction: PCR |
 90%
Level 1: 80%+
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The advantage of using DNA polymerases from thermophilic organisms in PCR is that:

1. the DNA polymerases of these bacteria are much faster than those from other organisms
2. the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
3. the DNA polymerases of these bacteria never make mistakes while replicating DNA
4. all of the above
Subtopic:  Process of Biotech |
 74%
Level 2: 60%+
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To isolate DNA from a cell, the cell wall needs to be broken. Match the enzymes in Column I with the type of cell in Column II and select the correct match from the codes given:
Column I Column II
A. Lysozyme P. Fungal cell
B. Cellulase Q. Plant cell
C. Chitinase R. Bacterial cell
Codes:
A B C
1. P Q R
2. P R Q
3. R P Q
4. R Q P
Subtopic:  Host & Desired DNA |
 92%
Level 1: 80%+
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Please attempt this question first.
In a continuous culture system:
I: The used medium is drained out from one side while the fresh medium is added from the other.
II: The cells are maintained in their physiologically most active log/exponential phase.
III: A larger biomass is produced leading to higher yields of desired proteins.
 
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
Subtopic:  Large Scale Production |
 88%
Level 1: 80%+
Please attempt this question first.
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Please attempt this question first.
Identify the correct statement:
1. DNA is the genetic material of all organisms without exception.
2. As it is an RNA world, the genetic material is ds RNA in majority of the organisms.
3. Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules.
4. Proteins such as histones can be removed from the cell lysate with the help of ribonuclease.
Subtopic:  Process of Biotech |
 78%
Level 2: 60%+
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Primers used in PCR are:
1. a complementary DNA sequence synthesised on the template of hnRNA
2. artificially synthesised or sequences used to induce replication of DNA
3. a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome
4. small chemically synthesised oligonucletides that are complementary to the regions of DNA
Subtopic:  Polymerase Chain Reaction: PCR |
 73%
Level 2: 60%+
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