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Q. No.If one needs to derive a cDNA of human insulin, which cells should be taken up for extraction?
1.
Any cell of the human body as the DNA is the same in all cells
2.
Any nucleated cell of the human body
3.
Beta cells of islets of Langerhans of the human pancreas
4.
WBCs
Subtopic: Med Applications & Humulin |
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To produce human recombinant insulin, Eli Lily:
| 1. | prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. |
| 2. | prepared two DNA sequences corresponding to A and C chains of human insulin and introduced them in plasmids of Yeast to produce insulin chains. |
| 3. | used reverse transcription of the hnRNA transcript of the insulin gene to make complementary DNA. |
| 4. | used fragments created by restriction enzyme digestion to isolate and used the gene for insulin from pancreatic cells. |
Subtopic: Med Applications & Humulin |
91%
From NCERT
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RNA interference:
| I: | involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing). |
| II: | has been used to create transgenic tobacco plants resistant to nematode Meloidogyne incognita |
| 1. | Only I is correct | 2. | Only II is correct |
| 3. | Both I and II are correct | 4. | Both I and II are incorrect |
Subtopic: Interference RNA Tech |
91%
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Consider the given two statements:
I. The first clinical gene therapy was given to a girl with adenosine deaminase deficiency
II. The first recombinant therapeutic product approved for human use was Human Insulin
I. The first clinical gene therapy was given to a girl with adenosine deaminase deficiency
II. The first recombinant therapeutic product approved for human use was Human Insulin
| 1. | Only I is correct | 2. | Only II is correct |
| 3. | Both I and II are correct | 4. | Both I and II are incorrect |
Subtopic: Gene Therapy |
88%
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With the help of ELISA, infection by pathogen can be detected by the presence in a sample of:
I. antigens of the pathogen
II. antibodies synthesized against the pathogen
I. antigens of the pathogen
II. antibodies synthesized against the pathogen
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
Subtopic: Molecular Diagnosis |
81%
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Biopiracy is:
| 1. | the exploration of natural sources for small molecules, macromolecules and biochemical and genetic information that could be developed into commercially valuable products. |
| 2. | exploitation of a region's biological resources or indigenous knowledge unethically and without providing fair compensation. |
| 3. | the use of living organisms, like microbes and bacteria, in the removal of contaminants, pollutants, and toxins from soil, water, and other environments |
| 4. | the process of developing a crop with bioavailable micronutrients in its edible parts. |
Subtopic: Ethical Issues |
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| Assertion(A): | Delivery of ADA cDNA into lymphocytes is not a permanent cure for ADA deficiency |
| Reason(R) | Lymphocytes do not express ADA gene. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of the (A) |
| 2. | Both (A) and (R) are True but (R) is not the correct explanation of the (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Gene Therapy |
60%
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| Assertion (A): | Transgenic animals can be specifically designed to allow the study of how genes are regulated, and how they affect the normal functions of the body and its development, e.g., study of complex factors involved in growth such as insulin-like growth factor. |
| Reason (R): | By introducing genes from other species that alter the formation of this factor and studying the biological effects that result, information is obtained about the biological role of the factor in the body. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are True but the (R) is not the correct explanation of (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Transgenic Microbes & Animals |
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| Assertion (A): | Whole plants can be regenerated from any part of a plant taken out and grown in a test tube, under sterile conditions in special nutrient media |
| Reason (R): | Every living plant cell is totipotent |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A) |
| 2. | Both (A) and (R) are True but the (R) is not the correct explanation of the (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Plant Tissue Culture |
67%
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A crop gets infected with a certain virus and the farmers of the area suffer immense financial loss. You have been asked to help the farmers and you decide to improve the crop by raising virus-free plants through plant tissue culture. The explant you will choose must be:
| 1. | Meristem | 2. | Anther |
| 3. | Embryo | 4. | Internode |
Subtopic: Plant Tissue Culture |
90%
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