Consider the following statements:
I: | Biolistics or gene gun can be used to introduce genes in both plant and animal cells. |
II: | Agrobacterium tumefaciens does not naturally infect Monocots. |
III: | Liposomes are used in gene therapy for cystic fibrosis. |
Which of the above statements are true?
1. | I and II only | 2. | I and III only |
3. | II and III only | 4. | I, II, and III |
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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
1. | Charge only | 2. | Size only |
3. | Charge-to-size ratio | 4. | All of the above |
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Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?
1. | restriction endonuclease digestion of cellular and plasmid DNAs. |
2. | production of recombinant DNA using DNA ligase and a mixture of digested cellular and plasmid DNAs. |
3. | separation of recombinant DNAs by electrophoresis using the Southern technique to determine where the desired recombinant migrates. |
4. | transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin. |
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DNA from a eukaryotic organism is digested with a restriction endonuclease and the resulting fragments are cloned into a plasmid vector. Bacteria transformed by these plasmids collectively contain all of the genes of the organism. This culture of bacteria is referred to as :
1. | restriction map | 2. | RFLP profile |
3. | F' factor | 4. | library |
Column I | Column II | ||
A | Lysozyme | P | Fungal cell |
B | Cellulase | Q | Plant cell |
C | Chitinase | R | Bacterial cell |
A | B | C | |
1. | P | Q | R |
2. | P | R | Q |
3. | R | P | Q |
4. | R | Q | P |