Identify a character that is not desirable in a cloning vector:
1. | an inactive promoter |
2. | an origin of the replication site |
3. | selectable markers such as genes for antibiotic resistance |
4. | one or more unique restriction endonuclease sites |
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The trait ‘resistance to certain antibiotics’ is seen in some bacteria due to the presence in them of:
1. | Inclusion bodies |
2. | Membranous extensions of plasma membrane |
3. | Plasmid DNA |
4. | Glycocalyx |
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Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
1. | Plastid | 2. | Bacteriophages |
3. | BAC | 4. | Plasmid |
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A piece of DNA is inserted within the gene of beta-galactosidase in the E.coli plasmid. X-gal is added to the medium during screening. The colonies of recombinant transformants:
1. | will show an increase in growth |
2. | will not be able to survive |
3. | will give a deep blue color |
4. | will not produce any color |
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In the diagram showing the plasmid pBR322, the recognition sequence of the restriction enzyme BamHI is shown by the number:
1. | 1 | 2. | 2 |
3. | 3 | 4. | 4 |
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Look at the given hypothetical vector-
Suppose we cut the desired gene as well as the plasmid with the help of the Restriction enzyme EcoR I and ligate them. What would be true?
1. | Kanamycin resistance will select the transformants from non-transformants while tetracycline resistance will select recombinant transformants from non-recombinant transformants. |
2. | Tetracyclin resistance will select the transformants from non-transformants while kanamycin resistance will select recombinant transformants from non-recombinant transformants. |
3. | Both will select only transformants. |
4. | Both will select only recombinants. |
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A major advantage of using YAC as a cloning vector over the plasmids is that:
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites
I: | Plasmid can be used as a vector in recombinant DNA technology experiments. |
II: | Plasmid is an extrachromosomal, circular, double-stranded DNA found in some bacteria. |
1. | Both I and II are correct and II explains I |
2. | Both I and II are correct but II does not explain I |
3. | I is correct but II is incorrect |
4. | Both I and II are incorrect |
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I: | The gene that was not inactivated helps in selecting the transformants. |
II: | The gene that was insertionally inactivated helps in selection of recombinants. |
1. | Only I is correct |
2. | Only II is correct |
3. | Both I and II are correct |
4. | I, II and III are correct |