- 1(current)
Q. No.DNA fragments resulting from restriction enzyme digestion are moved through agar gel during electrophoresis. Which of the following modifications to the fragments will not affect the rate of their movement?
1. Neutralizing the negative charge
2. Increasing length
3. Changing the base composition
4. Decreasing length
To isolate DNA in pure form from a bacterial cell, it should initially be treated with:
| 1. | Proteases and RNase but not DNase | 2. | Lysozyme |
| 3. | Restriction endonuclease | 4. | DNA ligase |
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Regarding competence for bacterial cell transformation:
| I: | Natural competence is not seen in bacterial cells as DNA is hydrophilic and a large molecule. |
| II: | Competence can be induced in bacterial cells with the help of divalent cations and brief heat shock. |
1. Only I is correct.
2. Only II is correct.
3. Both I and II are correct.
4. Both I and II are incorrect.
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| I: | Agar Gel electrophoresis is useful in analysis of DNA. |
| II: | DNA is generally positively charged due to its nitrogenous bases so an electrical field forces it to move toward a negative pole. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | I is correct but II is incorrect |
| 4. | Both I and II are incorrect |
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- 1(current)
Q. No.
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