- 1(current)
Q. No.Taq polymerase is used in the polymerase chain reaction because:
| 1. | It replicates DNA faster than other enzymes |
| 2. | It is the only enzyme that can replicate DNA invitro |
| 3. | It does not require an RNA primer to function |
| 4. | It is thermostable |
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Who among the following was awarded the Nobel Prize for the development of the PCR technique?
| 1. | Herbert Boyer | 2. | Hargovind Khurana |
| 3. | Kary Mullis | 4. | Arthur Kornberg |
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The enzyme used for DNA amplification in PCR procedures is:
1. Polynucleotide phosphorylase
2. Reverse transcriptase
3. Taq polymerase
4. DNA-dependent RNA polymerase
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The chronological steps of a polymerase chain reaction are:
| 1. | Denaturation, Annealing of primers, Primer extension |
| 2. | Annealing of primers, Denaturation, Primer extension |
| 3. | Annealing of primers, Primer extension, Denaturation |
| 4. | Denaturation, Primer extension, Annealing of primers |
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| 1. | For a cell to divide, it must first replicate its DNA. |
| 2. | DNA replication is an all-or-none process; once replication begins, it proceeds to completion. |
| 3. | DNA replication (DNA amplification) cannot be performed in vitro (artificially, outside a cell). |
| 4. | Once replication is complete, it does not occur again in the same cell cycle. |
| Assertion (A): | Thermostable DNA dependent DNA polymerase is used in Polymerase Chain Reaction |
| Reason (R): | Such enzymes do not require primers and can initiate DNA synthesis |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A) |
| 2. | Both (A) and (R) are True but the (R) is not the correct explanation of the (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
| I: | is used to synthesize multiple copies of the gene [or DNA] in vitro. |
| II: | uses a thermostable DNA dependent DNA polymerase. |
| III: | is now routinely used to detect HIV in suspected AIDS patient. |
| IV: | is being used to detect mutations in genes in suspected cancer patients. |
| 1. | All except I are true | 2. | Only II and III are true |
| 3. | Only IV is not true | 4. | I, II, III and IV are true |
- 1(current)
Q. No.
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