Q. No.In the first gene cloning experiment:
1.
Researchers successfully identified a human gene responsible for the disease.
2.
Researchers successfully inserted a gene for kanamycin resistance into a plasmid vector.
3.
Researchers demonstrated that many different DNA fragments could insert into a plasmid vector
4.
Researchers produced a strain of bacteriophage with an increased ability to infect E. coli.
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Which of the following would NOT be a useful selectable marker?
| 1. | A gene encoding a protein that degrades the antibiotic ampicillin. |
| 2. | A gene encoding a protein that allows the cell to synthesize histidine. |
| 3. | A gene encoding a protein that is an essential structural component of the cell. |
| 4. | All of these are useful selectable markers. |
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Consider the following statements:
| I: | Asexual reproduction preserves genetic information while sexual reproduction permits variations. |
| II: | Traditional hybridization often leads to the inclusion and multiplication of undesirable genes along with the desired genes. |
| III: | rDNA technology allows us to isolate and introduce only one or a set of desirable genes without introducing undesirable genes in the target organism. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
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Consider the following statements:
| I: | Exonucleases remove nucleotides from the ends of DNA whereas, endonucleases make cuts at specific positions within DNA. |
| II: | To visualize pure DNA fragments, it is stained with ethidium bromide and seen in UV light. |
| III: | Plasmids and phage DNA are used as vectors in genetic engineering as they are extrachromosomal DNA molecules. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
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Consider the following statements:
| I: | Ori is responsible for the copy number of the linked DNA. |
| II: | Transformation is a process through which a piece of DNA is introduced into a host bacterium. |
| III: | Bacteriophages have very high copy numbers of their genome within the bacterial cell. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
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Consider the following statements:
| I: | Biolistics or gene gun can be used to introduce genes in both plant and animal cells. |
| II: | Agrobacterium tumefaciens does not naturally infect Monocots. |
| III: | Liposomes are used in gene therapy for cystic fibrosis. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
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The bioreactor shown in the diagram is:
| 1. | Sparged stirred tank type | 2. | Airlift type |
| 3. | Simple stirred tank type | 4. | Fluidized bed type |
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You are inserting your gene of interest into the Lac Z gene in a plasmid also containing a tetracycline-resistant gene. You plate your transformed bacteria on media containing tetracycline and X-gal. Which of the following results would indicate a clone with recombinant plasmids?
| 1. | A clone which did not grow on the tetracycline plates |
| 2. | A white colony on the tetracycline plates |
| 3. | A blue colony on the tetracycline plates |
| 4. | A red colony on the tetracycline plates |
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What type of probe would you use for a Western blot experiment?
| 1. | A known DNA sequence. | 2. | An RNA molecule. |
| 3. | A purified protein. | 4. | An antibody. |
Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?
| 1. | restriction endonuclease digestion of cellular and plasmid DNAs. |
| 2. | production of recombinant DNA using DNA ligase and a mixture of digested cellular and plasmid DNAs. |
| 3. | separation of recombinant DNAs by electrophoresis using the Southern technique to determine where the desired recombinant migrates. |
| 4. | transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin. |
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