Q. No.Restriction enzymes do not act on the DNA of the host cell in which they are produced, because :
1.
Host DNA is packed into chromosomes
2.
Restriction enzymes are ineffective on host DNA
3.
Host DNA does not have the restriction site for the restriction enzymes.
4.
Restriction site of host DNA is methylated.
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Look at the given hypothetical vector-
Suppose we cut the desired gene as well as the plasmid with the help of the Restriction enzyme EcoR I and ligate them. What would be true?
| 1. | Kanamycin resistance will select the transformants from non-transformants while tetracycline resistance will select recombinant transformants from non-recombinant transformants. |
| 2. | Tetracyclin resistance will select the transformants from non-transformants while kanamycin resistance will select recombinant transformants from non-recombinant transformants. |
| 3. | Both will select only transformants. |
| 4. | Both will select only recombinants. |
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A piece of DNA is inserted within the gene of beta-galactosidase in the E.coli plasmid. X-gal is added to the medium during screening. The colonies of recombinant transformants:
| 1. | will show an increase in growth |
| 2. | will not be able to survive |
| 3. | will give a deep blue color |
| 4. | will not produce any color |
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It is useful to use restriction enzymes in rDNA experiments that produce sticky ends in the resultant fragments because:
| 1. | it allows a cell to recognize fragments produced by the enzyme |
| 2. | the single-stranded ends serve as starting points for DNA replication |
| 3. | the fragments will bond to other fragments with complementary single-stranded ends |
| 4. | only single-stranded DNA segments can code for proteins |
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Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
| 1. | Plastid | 2. | Bacteriophages |
| 3. | BAC | 4. | Plasmid |
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Which of the following cannot be used as a vector in rDNA technology?
| 1. | Plasmid | 2. | Phage DNA |
| 3. | Bacterium | 4. | YAC |
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In the diagram showing the plasmid pBR322, the recognition sequence of the restriction enzyme BamHI is shown by the number:
| 1. | 1 | 2. | 2 |
| 3. | 3 | 4. | 4 |
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Agrobacterium mediation cannot be used to introduce a gene of interest in which of the following plants?
| 1. | Pea | 2. | Tomato |
| 3. | Rice | 4. | Cotton |
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‘Downstream processing’ does not include:
| 1. | Product biosynthesis | 2. | Product isolation |
| 3. | Product purification | 4. | Product polishing |
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Who among the following was awarded the Nobel Prize for the development of the PCR technique?
| 1. | Herbert Boyer | 2. | Hargovind Khurana |
| 3. | Kary Mullis | 4. | Arthur Kornberg |
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