Q. No.Identify a character that is not desirable in a cloning vector:
1.
an inactive promoter
2.
an origin of the replication site
3.
selectable markers such as genes for antibiotic resistance
4.
one or more unique restriction endonuclease sites
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Restriction enzymes are synthesized by:
| 1. | bacteria only | 2. | yeast and bacteria |
| 3. | eukaryotic cells only | 4. | all kinds of cells |
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The first type II restriction endonuclease discovered that could cut dsDNA at a specific site was:
| 1. | EcoRI | 2. | SmaI |
| 3. | Hind II | 4. | Hind III |
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A danger of genetic engineering is that the genetically modified bacterium can escape the lab and infect humans. This possibility is prevented by:
| 1. | selecting a mutant form of the bacteria |
| 2. | selecting a live attenuated bacterium |
| 3. | application of stringent asepsis in the lab |
| 4. | prophylactically immunizing the persons working in the lab |
A DNA library is:
| 1. | information in a computer database about all the genes sequenced so far |
| 2. | a collection of vectors in a molecular biology lab |
| 3. | a collection of fragments of DNA that make up the entire genome of an organism |
| 4. | all the restriction sites put together on a DNA fragment |
A foreign DNA was introduced at a cloning site within the gene for beta-galactosidase in a plasmid of a bacterium. The bacterium containing this rDNA was plated on a medium containing X-gal. They were distinguished by the fact that they remained white. This method should be called as:
| 1. | selection | 2. | screening |
| 3. | transformation | 4. | transduction |
Cohen and Boyer constructed one of the first genetically engineered chimeras, pSC101. They introduced in this plasmid the gene of Xenopus laevis that coded for:
| 1. | cytochrome c | 2. | haemoglobin |
| 3. | keratin | 4. | rRNA |
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If the required gene has been isolated by shotgun approach, care must be taken to:
| 1. | cut the plasmid with a different restriction enzyme than that used to obtain the donor gene |
| 2. | cut the plasmid with the same restriction enzyme that was used to obtain the donor gene |
| 3. | avoid cutting the plasmid |
| 4. | cut the plasmid with two different restriction enzymes |
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The chronological steps of a polymerase chain reaction are:
| 1. | Denaturation, Annealing of primers, Primer extension |
| 2. | Annealing of primers, Denaturation, Primer extension |
| 3. | Annealing of primers, Primer extension, Denaturation |
| 4. | Denaturation, Primer extension, Annealing of primers |
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Taq polymerase is used in the polymerase chain reaction because:
| 1. | It replicates DNA faster than other enzymes |
| 2. | It is the only enzyme that can replicate DNA invitro |
| 3. | It does not require an RNA primer to function |
| 4. | It is thermostable |
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