- 1(current)
Q. No.In agarose gel electrophoresis, the DNA fragments produced by restriction enzyme digestion:
| 1. | will move towards anode with smaller fragments moving farther than the larger segments |
| 2. | will move towards anode with larger fragments moving farther than the smaller segments |
| 3. | will move towards cathode with smaller fragments moving farther than the larger segments |
| 4. | will move towards cathode with larger fragments moving farther than the smaller segments |
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| 1. | DNA is the genetic material of all organisms without exception. |
| 2. | As it is an RNA world, the genetic material is ds RNA in majority of the organisms. |
| 3. | Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules. |
| 4. | Proteins such as histones can be removed from the cell lysate with the help of ribonuclease. |
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Lysozyme should be used when isolating DNA in a pure form from:
| 1. | A bacterial cell | 2. | A fungal cell |
| 3. | A plant cell | 4. | An animal cell |
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While isolating DNA from bacteria, which of the following enzymes is not required?
| 1. | Lysozyme | 2. | Ribonuclease |
| 3. | Deoxyribonuclease | 4. | Protease |
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| Assertion (A): | Agar gel electrophoresis is employed to check the progression of a restriction enzyme digestion. |
| Reason (R): | Restriction enzyme digestion is performed by incubating purified DNA molecules with the restriction enzymes, at the optimal conditions for that specific enzyme. |
| 1. | Both (A) and (R) are False. |
| 2. | Both (A) and (R) are True but (R) does not explain (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are True and (R) explains (A). |
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| 1. | Lysozyme or Chitinase | 2. | DNase |
| 3. | DNAs | 4. | Proteases |
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Gene cloning occurs:
| 1. | when a phage transfers bacterial DNA from one bacterium to another. |
| 2. | when a bacterium takes up DNA from the surrounding fluid. |
| 3. | when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. |
| 4. | when DNA is produced from an RNA template. |
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Q. No.
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