I: | In the Cohen and Boyer experiment, the linking of antibiotic resistance gene with a plasmid vector became possible with the enzyme DNA ligase. |
II: | Recombinant DNA molecules are DNA molecules formed by laboratory methods of genetic recombination that bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome. |
1. | Only I |
2. | Only II |
3. | Both I and II |
4. | Neither I nor II |
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What is true regarding A, B and C in the given diagrammatic representation of rDNA technology?
I: | At A, same restriction enzyme is used to cut both foreign and vector DNA |
II: | The enzyme used at B is DNA ligase |
III: | Step C can be called as transformation |
1. | I and II only | 2. | I and III only |
3. | II and III only | 4. | I, II and III |
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Which of the following is not required in the preparation of a recombinant DNA molecule?
1. | Restriction endonuclease | 2. | DNA ligase |
3. | DNA fragments | 4. | E.coli |
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Lysozyme should be used when isolating DNA in a pure form from:
1. | A bacterial cell | 2. | A fungal cell |
3. | A plant cell | 4. | An animal cell |
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