Separation and Isolation of DNA fragments Biotechnology: Principles and Processes - Essentiale - (New NCERT) Biotechnology Principles and Processes Masterclass in Biology NEET Practice Questions, MCQs, Past Year Questions (PYQs), NCERT Questions, Question Bank, Class 11 and Class 12 Questions, and PDF solved with answers

DNA fragments digested by restriction endonucleases separate on agar gel electrophoresis according to their size because:

1.	they are forced to move towards cathode under an electric field
2.	agar gel provides a sieving effect
3.	larger fragments will have more negative charge and will move farther in the electric field
4.	chelation with ethidium bromide reduce the rate of transport of fragments

Subtopic: Separation and Isolation of DNA fragments |

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While isolating DNA from bacteria, which of the following enzymes is not required?

1.	Lysozyme	2.	Ribonuclease
3.	Deoxyribonuclease	4.	Protease

Subtopic: Process of Biotech | Separation and Isolation of DNA fragments | Tools |

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