Q. No.The term ‘molecular scissors’ is used for:
1.
restriction enzymes
2.
Taq polymerase
3.
reverse transcriptase
4.
DNA ligase
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| I: | In the Cohen and Boyer experiment, the linking of antibiotic resistance gene with a plasmid vector became possible with the enzyme DNA ligase. |
| II: | Recombinant DNA molecules are DNA molecules formed by laboratory methods of genetic recombination that bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome. |
| 1. | Only Statement I |
| 2. | Only Statement II |
| 3. | Both Statement I and Statement II |
| 4. | Neither Statement I nor Statement II |
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| Statement I: | In 1963, two enzymes responsible for restricting the growth of bacteriophage in E.coli were isolated. |
| Statement II: | In 1968, the first restriction endonuclease, that always cut DNA molecules by recognising a specific sequence six base pairs, was isolated and characterised. |
| 1. | Only Statement I |
| 2. | Only Statement II |
| 3. | Both Statement I and Statement II |
| 4. | Neither Statement I nor Statement II |
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| I: | The enzyme names begin with an italicized three-letter acronym; the first letter of the acronym is the first letter of the genus of bacteria from which the enzyme was isolated, the next two letters are the two letters of the species. |
| II: | These are followed by extra letters or numbers to indicate the serotype or strain, a space, then a Roman numeral to indicate the chronology of identification. |
| 1. | Only I is correct | 2. | Only II is correct |
| 3. | Both I and II are correct | 4. | Both I and II are incorrect |
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| 1. | that reads same on the two strands when orientation of reading is kept the same. |
| 2. | that reads same on the two strands when orientation of reading is 5’ to 3’ on one end and 3’ to 5’ on the other. |
| 3. | that are complementary on the two strands when orientation of reading is kept the same. |
| 4. | that are complementary on the two strands when orientation of reading is 5’ to 3’ on one end and 3’ to 5’ on the other. |
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| I. | Bacteriophages because of their high number per cell, have very high copy numbers of their genome within the bacterial cells. |
| II. | If one wants to recover many copies of the target DNA, it should be cloned in a vector whose origin supports high copy number. |
| III. | A selectable marker helps in identifying and eliminating non-transformants and selectively permits the growth of the transformants. |
| IV. | Ti plasmid of Agrobacterium tumefaciens has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use mechanisms to deliver genes of our interest into a variety of plants. |
| V. | Retroviruses in animals have the ability to transform normal cells into cancerous cells and hence can never be used as a cloning vector in rDNA procedures. |
| 1. | 2 | 2. | 3 |
| 3. | 4 | 4. | 5 |
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| Assertion (A): | In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes: |
| Reason: (R): | Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. |
| 1. | Both (A) and (R) are true and (R) explains (A). |
| 2. | (A) is true but (R) is false. |
| 3. | Both (A) and (R) are true but (R) does not explain (A). |
| 4. | Both (A) and (R) are false. |
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| 1. | 27°C | 2. | 42°C |
| 3. | 72°C | 4. | 98°C |
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| 1. | DNA is the genetic material of all organisms without exception. |
| 2. | As it is an RNA world, the genetic material is ds RNA in majority of the organisms. |
| 3. | Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules. |
| 4. | Proteins such as histones can be removed from the cell lysate with the help of ribonuclease. |
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| Assertion (A): | Agar gel electrophoresis is employed to check the progression of a restriction enzyme digestion. |
| Reason (R): | Restriction enzyme digestion is performed by incubating purified DNA molecules with the restriction enzymes, at the optimal conditions for that specific enzyme. |
| 1. | Both (A) and (R) are False. |
| 2. | Both (A) and (R) are True but (R) does not explain (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are True and (R) explains (A). |
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