Q. No.Bioprocessing engineering deals with:
1.
the search for plant and animal species from which medicinal drugs and other commercially valuable compounds can be obtained.
2.
developing methods and software tools for understanding biological data.
3.
production of active pharmaceutical substances in genetically modified organisms (GMOs).
4.
maintenance of sterile ambience in chemical engineering processes.
Identify the incorrect statement regarding restriction endonucleases:
| 1. | They mostly cut dsDNA at specific base sequences. |
| 2. | They are produced by bacterial cells as a mechanism of self-defence. |
| 3. | They digest DNA by removing nucleotides from a free 3' end. |
| 4. | They often generate short single-stranded sequences at the ends of the resultant fragments. |
A donor DNA and plasmid vector DNA of E. coli are cut by EcoRI. The plasmid contains genes for resistance to ampicillin and tetracycline. The ECoRI recognition sequence lies within the gene for tetracycline resistance. Ligase is used, and the recombinant DNA is produced. The plasmids are transferred to E.coli with the help of electroporation. Samples of the bacterial colonies are then grown in four different media: nutrient medium plus ampicillin, nutrient medium plus tetracycline, nutrient medium plus ampicillin and tetracycline, and nutrient medium without antibiotics. Non-recombinant transformants will grow on:
| 1. | the nutrient medium plus ampicillin, but not on the medium containing tetracycline. |
| 2. | only on the medium containing both antibiotics. |
| 3. | on the medium containing tetracycline, but not on the medium containing ampicillin. |
| 4. | on all four types of mediums. |
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If we are able to link an alien piece of DNA with bacteriophage or plasmid DNA, we can multiply its number:
| 1. | more than the number of copy number of bacteriophages in the cells and equal to the copy number of plasmid in the cell. |
| 2. | equal to the number of copy number of bacteriophages in the cells and equal to the copy number of plasmid in the cell. |
| 3. | equal to the number of copy number of bacteriophages in the cells and less than the copy number of plasmid in the cell. |
| 4. | less than the number of copy number of bacteriophages in the cells and more than the copy number of plasmid in the cell. |
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The Ti plasmid, used in transformation of plant cells, is found in:
| 1. | Agrobacterium tumefaciens | 2. | Escherichia coli |
| 3. | Bacillus thuringiensis | 4. | Xanthomonas citri |
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Which of the following can be used to induce competence for getting a bacterial cell transformed during rDNA procedures?
| 1. | A divalent cation | 2. | Ethidium bromide |
| 3. | Polyethylene glycol | 4. | Anionic proteins |
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In microinjection, the rDNA :
| 1. | coats the micropellets and is used in biolistics to transform plant cells |
| 2. | is directly injected into the cytoplasm of a plant or an animal cell |
| 3. | is delivered in the vicinity of the plasma membrane of the cell (to be transformed) |
| 4. | is directly injected into the nucleus of an animal cell |
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The downstream processing stage of rDNA does not involve:
| 1. | Biosynthesis | 2. | Separation |
| 3. | Purification | 4. | Preservation |
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Some bacteria are resistant to antibiotics. This trait is normally due to:
1. lack of membrane bound organelles
2. presence of a cell wall
3. presence of an extra-chromosomal circular DNA
4. a unique feature called as inclusion bodies
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Agrobacterium tumefaciens can be an effective vector for delivering genes of interest into the cells of:
| 1. | wheat | 2. | soybean |
| 3. | rice | 4. | barley |
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