Q. No.The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?
1.
Banting and Best
2.
Bolivar and Rodriguez
3.
Hershey and Chase
4.
Cohen and Boyer
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The construction of first rDNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of:
| 1. | Salmonella typhimurium | 2. | Escherechia coli |
| 3. | Agrobacterium tumefaciens | 4. | Haemophilus influenzae |
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The ability of plasmids and bacteriophage DNA to replicate within bacterial cells:
| 1. | depends on a particular or sequence of the main chromosomal DNA |
| 2. | is possible due to promoter sequence present in the insert |
| 3. | cannot occur without telomeric sequences |
| 4. | is independent of the control of chromosomal DNA |
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What controls the copy number of linked DNA if plasmid is used as a vector in bacterial transformation?
| 1. | Ori | 2. | rop |
| 3. | Selectable markers | 4. | Promoter in the insert |
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In the given diagram of pBR322, the location of the recognition sequences of BamH I and Sal I are represented by the numbers:
|
 |
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In agarose gel electrophoresis, the DNA fragments produced by restriction enzyme digestion:
| 1. | will move towards anode with smaller fragments moving farther than the larger segments |
| 2. | will move towards anode with larger fragments moving farther than the smaller segments |
| 3. | will move towards cathode with smaller fragments moving farther than the larger segments |
| 4. | will move towards cathode with larger fragments moving farther than the smaller segments |
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If you want to isolate DNA in pure form from a fungal cell, the first enzyme required normally would be:
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA polymerase | 4. | Chitinase |
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A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:
| 1. | It includes the centromere |
| 2. | It becomes a part of chromosome |
| 3. | It does not contain any intron sequences |
| 4. | It is a single stranded polynucleotide |
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What limitation of traditional hybridization procedures used in plant and animal breeding can be overcome by recombinant DNA procedures?
| I: | Inclusion and multiplication of undesirable genes |
| II: | Inability of inclusion of desirable traits from other species |
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
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A reporter enzyme used in biotechnology includes all the following except:
1. Green Fluorescent Protein
2. Luciferase
3.Beta Galactosidase
4. Chloramphenicol acetyl transferase
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