- 1(current)
Q. No.The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?
| 1. | Banting and Best | 2. | Bolivar and Rodriguez |
| 3. | Hershey and Chase | 4. | Cohen and Boyer |
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| 1. | that reads same on the two strands when orientation of reading is kept the same. |
| 2. | that reads same on the two strands when orientation of reading is 5’ to 3’ on one end and 3’ to 5’ on the other. |
| 3. | that are complementary on the two strands when orientation of reading is kept the same. |
| 4. | that are complementary on the two strands when orientation of reading is 5’ to 3’ on one end and 3’ to 5’ on the other. |
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Identify the incorrect statement regarding restriction endonucleases:
| 1. | They mostly cut dsDNA at specific base sequences. |
| 2. | They are produced by bacterial cells as a mechanism of self-defence. |
| 3. | They digest DNA by removing nucleotides from a free 3' end. |
| 4. | They often generate short single-stranded sequences at the ends of the resultant fragments. |
| I: | The enzyme names begin with an italicized three-letter acronym; the first letter of the acronym is the first letter of the genus of bacteria from which the enzyme was isolated, the next two letters are the two letters of the species. |
| II: | These are followed by extra letters or numbers to indicate the serotype or strain, a space, then a Roman numeral to indicate the chronology of identification. |
| 1. | Only I is correct | 2. | Only II is correct |
| 3. | Both I and II are correct | 4. | Both I and II are incorrect |
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of the phosphodiester bonds in DNA by the enzyme |
| 2. | Cutting of DNA at a specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Consider the two statements:
| I: | It is desirable to use restriction enzymes produce 'sticky ends' for cloning experiments in genetic engineering. |
| II: | If the donor DNA and the vector DNA are cut by such same restriction endonuclease, ligation of the two is easy. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | Only I is correct |
| 4. | Both I and II are incorrect |
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Lysozyme should be used when isolating DNA in a pure form from:
| 1. | A bacterial cell | 2. | A fungal cell |
| 3. | A plant cell | 4. | An animal cell |
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- 1(current)
Q. No.
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