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Q. No.Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
| 1. | DNA can be seen in visible light |
| 2. | DNA can be seen without staining in visible light |
| 3. | Ethidium bromide stained DNA can be seen in visible light |
| 4. | Ethidium bromide stained DNA can be seen under exposure to UV light |
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| 1. | a complementary DNA sequence synthesised on the template of hnRNA |
| 2. | artificially synthesised or sequences used to induce replication of DNA |
| 3. | a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome |
| 4. | small chemically synthesised oligonucletides that are complementary to the regions of DNA |
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The underlying basis of the Polymerase Chain Reaction is:
| 1. | DNA synthesis and hence amplification can occur in a template independent manner. |
| 2. | DNA, being thermostable, can be amplified during the thermal cycle in PCR. |
| 3. | DNA polymerase I can synthesize large amounts of DNA. |
| 4. | PCR allows the direct amplification of DNA even if it is present in minute quantities. |
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The steps included in a cycle of Polymerase Chain Reaction are:
1. initiation, elongation, and termination
2. transfection, transformation, and transduction
3. denaturation, annealing, and extension
4. restriction, electrophoresis, and elution
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The enzyme used in the polymerase chain reaction is a:
1. DNA dependent RNA polymerase
2. RNA dependent DNA polymerase
3. DNA dependent DNA polymerase
4. RNA dependent RNA polymerase
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Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
1. Denaturation of template DNA
2. Annealing of primers to template DNA
3. Extension of primer end on the template DNA
4. All of the above
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Q. No.
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