Q. No.The palindrome in DNA is a sequence of base pairs:
1.
that reads same on the two strands when orientation of reading is kept the same.
2.
that reads same on the two strands when orientation of reading is 5’ to 3’ on one end and 3’ to 5’ on the other.
3.
that are complementary on the two strands when orientation of reading is kept the same.
4.
that are complementary on the two strands when orientation of reading is 5’ to 3’ on one end and 3’ to 5’ on the other.
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| 1. | Lysozyme or Chitinase | 2. | DNase |
| 3. | DNAs | 4. | Proteases |
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| 1. | a complementary DNA sequence synthesised on the template of hnRNA |
| 2. | artificially synthesised or sequences used to induce replication of DNA |
| 3. | a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome |
| 4. | small chemically synthesised oligonucletides that are complementary to the regions of DNA |
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Elution is:
| 1. | separating the restricted DNA fragments on agarose gel |
| 2. | staining the separated DNA fragments with ethidium bromide |
| 3. | cutting out of the separated bands of DNA from the agarose gel and extracting them from the gel piece |
| 4. | constructing rDNA by joining the purified DNA fragments to the cloning vector |
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A cloning vector has two antibiotic resistance genes: tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinant transformants would survive on the medium containing:
1. ampicillin but not tetracycline
2. tetracycline but not ampicillin
3. both tetracycline and ampicillin
4. neither tetracycline nor ampicillin
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| I: | The enzyme names begin with an italicized three-letter acronym; the first letter of the acronym is the first letter of the genus of bacteria from which the enzyme was isolated, the next two letters are the two letters of the species. |
| II: | These are followed by extra letters or numbers to indicate the serotype or strain, a space, then a Roman numeral to indicate the chronology of identification. |
| 1. | Only I is correct | 2. | Only II is correct |
| 3. | Both I and II are correct | 4. | Both I and II are incorrect |
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| 1. | The use of living cells and bacteria in industrial and scientific processes. |
| 2. | The integration of natural sciences and organisms, cells, parts thereof, and molecular analogues for products and services. |
| 3. | The use of biology to solve problems and make useful products. |
| 4. | The use of biology to develop new products, methods and organisms intended to improve human health and society. |
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DNA fragments digested by restriction endonucleases separate on agar gel electrophoresis according to their size because:
| 1. | they are forced to move towards cathode under an electric field |
| 2. | agar gel provides a sieving effect |
| 3. | larger fragments will have more negative charge and will move farther in the electric field |
| 4. | chelation with ethidium bromide reduce the rate of transport of fragments |
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The underlying basis of the Polymerase Chain Reaction is:
| 1. | DNA synthesis and hence amplification can occur in a template independent manner. |
| 2. | DNA, being thermostable, can be amplified during the thermal cycle in PCR. |
| 3. | DNA polymerase I can synthesize large amounts of DNA. |
| 4. | PCR allows the direct amplification of DNA even if it is present in minute quantities. |
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Plasmid pUC18 carries lacZ gene that confers the host cells to convert white X-gal into blue indole. A gene is inserted within the lacZ gene of the plasmid. Bacterial cells transformed with this rDNA, if provided with X-gal in the medium, will produce:
| 1. | Blue colonies |
| 2. | White colonies |
| 3. | No colonies |
| 4. | Orange colonies if seen through UV light |
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