Q. No.Bioprocessing engineering deals with:
1.
the search for plant and animal species from which medicinal drugs and other commercially valuable compounds can be obtained.
2.
developing methods and software tools for understanding biological data.
3.
production of active pharmaceutical substances in genetically modified organisms (GMOs).
4.
maintenance of sterile ambience in chemical engineering processes.
Identify the incorrect statement regarding restriction endonucleases:
| 1. | They mostly cut dsDNA at specific base sequences. |
| 2. | They are produced by bacterial cells as a mechanism of self-defence. |
| 3. | They digest DNA by removing nucleotides from a free 3' end. |
| 4. | They often generate short single-stranded sequences at the ends of the resultant fragments. |
The Ti plasmid, used in transformation of plant cells, is found in:
| 1. | Agrobacterium tumefaciens | 2. | Escherichia coli |
| 3. | Bacillus thuringiensis | 4. | Xanthomonas citri |
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Which of the following can be used to induce competence for getting a bacterial cell transformed during rDNA procedures?
| 1. | A divalent cation | 2. | Ethidium bromide |
| 3. | Polyethylene glycol | 4. | Anionic proteins |
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The downstream processing stage of rDNA does not involve:
| 1. | Biosynthesis | 2. | Separation |
| 3. | Purification | 4. | Preservation |
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Some bacteria are resistant to antibiotics. This trait is normally due to:
1. lack of membrane bound organelles
2. presence of a cell wall
3. presence of an extra-chromosomal circular DNA
4. a unique feature called as inclusion bodies
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Agrobacterium tumefaciens can be an effective vector for delivering genes of interest into the cells of:
| 1. | wheat | 2. | soybean |
| 3. | rice | 4. | barley |
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The steps included in a cycle of Polymerase Chain Reaction are:
1. initiation, elongation, and termination
2. transfection, transformation, and transduction
3. denaturation, annealing, and extension
4. restriction, electrophoresis, and elution
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Consider the two statements:
| I: | It is desirable to use restriction enzymes produce 'sticky ends' for cloning experiments in genetic engineering. |
| II: | If the donor DNA and the vector DNA are cut by such same restriction endonuclease, ligation of the two is easy. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | Only I is correct |
| 4. | Both I and II are incorrect |
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Plasmid pUC18 carries lacZ gene that confers the host cells to convert white X-gal into blue indole. A gene is inserted within the lacZ gene of the plasmid. Bacterial cells transformed with this rDNA, if provided with X-gal in the medium, will produce:
| 1. | Blue colonies |
| 2. | White colonies |
| 3. | No colonies |
| 4. | Orange colonies if seen through UV light |
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