Q. No.Which of the enzymes whose recognition sites are shown here would produce sticky ends compatible with BstAUI (5'-G|GTACC-3')?
1. Bcl I (5'-T|GATCA-3')
2. Sun I (5'-C|GTACG-3')
3. Hind III (5'-A|AGCTT-3')
4. Kpn I (5'-GGTAC|C-3')
Identify the incorrectly matched pair:
restriction enzyme recognition site
1. EcoRI 5’GAATTC3’
3’CTTAAG5’
2. HindII 5’GTPyPuAC3’
3’CAPuPyTG3’
3. SmaI 5’GGATTC3’
3’CTTAGG5’
4. HindIII 5’AAGCTT3’
3’TTCGAA5’
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Identify the wrong statement with regards to restriction enzyme:
| 1. | They cut the strength at palindromic sites. |
| 2. | They are useful in genetic engineering. |
| 3. | Sticky ends can be joined by using DNA ligases. |
| 4. | Each restriction enzyme functions by inspecting the length of a DNA sequence. |
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Bacteria can protect themselves from getting infected by bacteriophages as they synthesize:
1. Methylase
2. Ligases
3. Plasmids
4. Endonucleases
Read the following four statements (A-D) about certain mistakes in two of them
(A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactal bumin enriched.
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules.
(C) Downstream processing is one of the steps of rDNA technology.
(D) Disarmed pathogen vectors are also used in transfer of r-DNA into the host.
Which are the two statements having mistakes?
1. Statement (B) and (C)
2. Statement (C) and (D)
3. Statement (A) and (C)
4. Statement (A) and (B)
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Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
1. 5’______CACGTA_______3’
3’______CTVAGT_______5’
2. 5’______CGTTCG_______3’
3’______ATGGTA_______5’
3. 5’______GATATG_______3’
3’______CTACTA_______5’
4. 5’______GAATTC_______3’
3’______CTTAAG_______5’
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Which one of the following represents a palindromic sequence in DNA?
1. 5' - GAATTC - 3'
3' - CTTAAG - 5'
2. 5' - CCAATG - 3'
3' - GAATCC - 5'
3. 5' - CATTAG - 3'
3' - GATAAC - 5'
4. 5' - GATACC - 3'
3' - CCTAAG - 5'
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There is a restriction endonuclease called EcoRI. What does ‘co’ part in it stand for?
1. Colon
2. Coelom
3. Coenzyme
4. coli
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Restriction endonucleases are enzymes which
1. Remove nucleotides from the ends of the DNA molecule.
2. Make cuts at specific positions within the DNA molecule.
3. Recognize a specific nucleotide sequence for binding of DNA ligase.
4. Restrict the action of the enzyme DNA polymerase.
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Restriction endonuclease
1. Cuts the DNA molecule randomly
2. Cuts the DNA molecule at specific sites
3. Restricts the synthesis of DNA inside the nucleus.
4. Synthesizes DNA.
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