- 1(current)
Q. No.If the required gene has been isolated by shotgun approach, care must be taken to:
| 1. | cut the plasmid with a different restriction enzyme than that used to obtain the donor gene |
| 2. | cut the plasmid with the same restriction enzyme that was used to obtain the donor gene |
| 3. | avoid cutting the plasmid |
| 4. | cut the plasmid with two different restriction enzymes |
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A foreign DNA was introduced at a cloning site within the gene for beta-galactosidase in a plasmid of a bacterium. The bacterium containing this rDNA was plated on a medium containing X-gal. They were distinguished by the fact that they remained white. This method should be called as:
| 1. | selection | 2. | screening |
| 3. | transformation | 4. | transduction |
An antibiotic resistance gene in a vector usually helps in the selection of:
1. Competent bacterial cells
2. Transformed bacterial cells
3. Recombinant bacterial cells
4. None of the above
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Consider the two statements:
| I: | It is beneficial to have a multiple cloning site (MCS) within the lac Z gene of the plasmid of E.coli. |
| II: | This allows the researcher to distinguish between recombinant and non-recombinant vectors if X-gal is added to the medium. |
1. Both I and II are correct and II explains I.
2. Both I and II are correct but II does not explain I.
3. I is correct but II is incorrect.
4. Both I and II are incorrect.
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- 1(current)
Q. No.
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