Q. No.‘Downstream processing’ does not include:
1. Product biosynthesis 2. Product isolation 3. Product purification 4. Product polishing
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The trait ‘resistance to certain antibiotics’ is seen in some bacteria due to the presence in them of:
| 1. | Inclusion bodies |
| 2. | Membranous extensions of plasma membrane |
| 3. | Plasmid DNA |
| 4. | Glycocalyx |
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Consider the two statements:
| I: | It is beneficial to have a multiple cloning site (MCS) within the lac Z gene of the plasmid of E.coli. |
| II: | This allows the researcher to distinguish between recombinant and non-recombinant vectors if X-gal is added to the medium. |
1. Both I and II are correct and II explains I.
2. Both I and II are correct but II does not explain I.
3. I is correct but II is incorrect.
4. Both I and II are incorrect.
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Agrobacterium mediation cannot be used to introduce a gene of interest in which of the following plants?
| 1. | Pea | 2. | Tomato |
| 3. | Rice | 4. | Cotton |
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In the diagram showing the plasmid pBR322, the recognition sequence of the restriction enzyme BamHI is shown by the number:
| 1. | 1 | 2. | 2 |
| 3. | 3 | 4. | 4 |
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A piece of DNA is inserted within the gene of beta-galactosidase in the E.coli plasmid. X-gal is added to the medium during screening. The colonies of recombinant transformants:
| 1. | will show an increase in growth |
| 2. | will not be able to survive |
| 3. | will give a deep blue color |
| 4. | will not produce any color |
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Look at the given hypothetical vector-
Suppose we cut the desired gene as well as the plasmid with the help of the Restriction enzyme EcoR I and ligate them. What would be true?
| 1. | Kanamycin resistance will select the transformants from non-transformants while tetracycline resistance will select recombinant transformants from non-recombinant transformants. |
| 2. | Tetracyclin resistance will select the transformants from non-transformants while kanamycin resistance will select recombinant transformants from non-recombinant transformants. |
| 3. | Both will select only transformants. |
| 4. | Both will select only recombinants. |
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Restriction enzymes do not act on the DNA of the host cell in which they are produced, because :
| 1. | Host DNA is packed into chromosomes |
| 2. | Restriction enzymes are ineffective on host DNA |
| 3. | Host DNA does not have the restriction site for the restriction enzymes. |
| 4. | Restriction site of host DNA is methylated. |
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It is useful to use restriction enzymes in rDNA experiments that produce sticky ends in the resultant fragments because:
| 1. | it allows a cell to recognize fragments produced by the enzyme |
| 2. | the single-stranded ends serve as starting points for DNA replication |
| 3. | the fragments will bond to other fragments with complementary single-stranded ends |
| 4. | only single-stranded DNA segments can code for proteins |
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Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
| 1. | Plastid | 2. | Bacteriophages |
| 3. | BAC | 4. | Plasmid |
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