Q. No.The first type II restriction endonuclease discovered that could cut dsDNA at a specific site was:
1.
EcoRI
2.
SmaI
3.
Hind II
4.
Hind III
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A danger of genetic engineering is that the genetically modified bacterium can escape the lab and infect humans. This possibility is prevented by:
| 1. | selecting a mutant form of the bacteria |
| 2. | selecting a live attenuated bacterium |
| 3. | application of stringent asepsis in the lab |
| 4. | prophylactically immunizing the persons working in the lab |
A DNA library is:
| 1. | information in a computer database about all the genes sequenced so far |
| 2. | a collection of vectors in a molecular biology lab |
| 3. | a collection of fragments of DNA that make up the entire genome of an organism |
| 4. | all the restriction sites put together on a DNA fragment |
A foreign DNA was introduced at a cloning site within the gene for beta-galactosidase in a plasmid of a bacterium. The bacterium containing this rDNA was plated on a medium containing X-gal. They were distinguished by the fact that they remained white. This method should be called as:
| 1. | selection | 2. | screening |
| 3. | transformation | 4. | transduction |
Cohen and Boyer constructed one of the first genetically engineered chimeras, pSC101. They introduced in this plasmid the gene of Xenopus laevis that coded for:
| 1. | cytochrome c | 2. | haemoglobin |
| 3. | keratin | 4. | rRNA |
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If the required gene has been isolated by shotgun approach, care must be taken to:
| 1. | cut the plasmid with a different restriction enzyme than that used to obtain the donor gene |
| 2. | cut the plasmid with the same restriction enzyme that was used to obtain the donor gene |
| 3. | avoid cutting the plasmid |
| 4. | cut the plasmid with two different restriction enzymes |
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The chronological steps of a polymerase chain reaction are:
| 1. | Denaturation, Annealing of primers, Primer extension |
| 2. | Annealing of primers, Denaturation, Primer extension |
| 3. | Annealing of primers, Primer extension, Denaturation |
| 4. | Denaturation, Primer extension, Annealing of primers |
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Taq polymerase is used in the polymerase chain reaction because:
| 1. | It replicates DNA faster than other enzymes |
| 2. | It is the only enzyme that can replicate DNA invitro |
| 3. | It does not require an RNA primer to function |
| 4. | It is thermostable |
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A major advantage of using YAC as a cloning vector over the plasmids is that:
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites
Which of the following enzymes catalyze the removal of nucleotides from the ends of DNA?
| 1. | endonuclease | 2. | exonuclease |
| 3. | DNA ligase | 4. | Hind – II |
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