Q. No.The number of correct statements regarding cloning vectors amongst the given statements is:
I.
Bacteriophages because of their high number per cell, have very high copy numbers of their genome within the bacterial cells.
II.
If one wants to recover many copies of the target DNA, it should be cloned in a vector whose origin supports high copy number.
III.
A selectable marker helps in identifying and eliminating non-transformants and selectively permits the growth of the transformants.
IV.
Ti plasmid of Agrobacterium tumefaciens has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use mechanisms to deliver genes of our interest into a variety of plants.
V.
Retroviruses in animals have the ability to transform normal cells into cancerous cells and hence can never be used as a cloning vector in rDNA procedures.
1.
2
2.
3
3.
4
4.
5
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| Assertion (A): | In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes: |
| Reason: (R): | Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. |
| 1. | Both (A) and (R) are true and (R) explains (A). |
| 2. | (A) is true but (R) is false. |
| 3. | Both (A) and (R) are true but (R) does not explain (A). |
| 4. | Both (A) and (R) are false. |
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| 1. | 27°C | 2. | 42°C |
| 3. | 72°C | 4. | 98°C |
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| 1. | DNA is the genetic material of all organisms without exception. |
| 2. | As it is an RNA world, the genetic material is ds RNA in majority of the organisms. |
| 3. | Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules. |
| 4. | Proteins such as histones can be removed from the cell lysate with the help of ribonuclease. |
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| Assertion (A): | Agar gel electrophoresis is employed to check the progression of a restriction enzyme digestion. |
| Reason (R): | Restriction enzyme digestion is performed by incubating purified DNA molecules with the restriction enzymes, at the optimal conditions for that specific enzyme. |
| 1. | Both (A) and (R) are False. |
| 2. | Both (A) and (R) are True but (R) does not explain (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are True and (R) explains (A). |
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| Assertion (A): | Bioreactors are important in biotechnology procedures to obtain desired products. |
| Reason (R): | Bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions |
| 1. | Both (A) and (R) are false. |
| 2. | Both (A) and (R) are true and (R) explains (A). |
| 3. | Both (A) and (R) are true but (R) does not explain (A). |
| 4. | (A) is true but (R) is false. |
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| 1. | Lysozyme or Chitinase | 2. | DNase |
| 3. | DNAs | 4. | Proteases |
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| 1. | a complementary DNA sequence synthesised on the template of hnRNA |
| 2. | artificially synthesised or sequences used to induce replication of DNA |
| 3. | a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome |
| 4. | small chemically synthesised oligonucletides that are complementary to the regions of DNA |
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| Assertion (A): | It is possible to select a bacterial cell transformed with a recombinant DNA bearing gene for an antibiotic resistance if the transformed cells are spread on agar plates containing the antibiotic. |
| Reason (R): | Due to insertional inactivation of the gene for antibiotic resistance gene, only the untransformed bacterial cells will grow and the transformed bacterial cells will die. |
| 1. | Both (A) and (R) are false. |
| 2. | Both (A) and (R) are true and (R) explains (A). |
| 3. | (A) is true but (R) is false. |
| 4. | Both (A) and (R) are true but (R) does not explain (A). |
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| Assertion (A): | There is a need for the recombinant DNA to be expressed in recombinant DNA technology procedures. |
| Reason (R): | In almost all recombinant DNA technologies, the ultimate aim is to produce a desirable protein. |
| 1. | Both (A) and (R) are False. |
| 2. | Both (A) and (R) are True and (R) explains (A). |
| 3. | Both (A) and (R) are True but (R) does not explain (A). |
| 4. | (A) is True but (R) is False. |
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