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Q. No.What is true regarding Downstream Processing?
I. This stage is after the biosynthetic stage.
II. The processes include separation and purification.
1. Only I 2. Only II 3. Both I and II 4. Neither I nor II
Subtopic: Large Scale Production |
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In 1970, Hamilton O. Smith, Thomas Kelly and Kent Wilcox isolated and characterized the first type II restriction enzyme that was:
| 1. | EcoR I | 2. | Hind II |
| 3. | Bam HI | 4. | Sma I |
Subtopic: Restriction Enzymes - Main Enzymes |
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In a continuous culture system:
| I: | The used medium is drained out from one side while the fresh medium is added from the other. |
| II: | The cells are maintained in their physiologically most active log/exponential phase. |
| III: | A larger biomass is produced leading to higher yields of desired proteins. |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II and III are correct |
Subtopic: Large Scale Production |
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A plasmid contains two genes for resistance to separate antibiotics. One of the two is insertionally inactivated due to insertion of alien DNA.
| I: | The gene that was not inactivated helps in selecting the transformants. |
| II: | The gene that was insertionally inactivated helps in selection of recombinants. |
| 1. | Only I is correct |
| 2. | Only II is correct |
| 3. | Both I and II are correct |
| 4. | I, II and III are correct |
Subtopic: Cloning Vector | Selection of Recombinant Transformants |
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The polymerase chain reaction:
| I: | is used to synthesize multiple copies of the gene [or DNA] in vitro. |
| II: | uses a thermostable DNA dependent DNA polymerase. |
| III: | is now routinely used to detect HIV in suspected AIDS patient. |
| IV: | is being used to detect mutations in genes in suspected cancer patients. |
| 1. | All except I are true | 2. | Only II and III are true |
| 3. | Only IV is not true | 4. | I, II, III and IV are true |
Subtopic: Polymerase Chain Reaction: PCR |
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Animal cells cannot be transformed with the help of:
| 1. | Microinjection | 2. | Ti Plasmid |
| 3. | Retroviral vectors | 4. | Liposomes |
Subtopic: Transforming Plant & Animal Cell |
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Plasmids are good cloning vectors mainly because:
| 1. | they are circular |
| 2. | they replicate autonomously |
| 3. | they are extra-chromosomal DNA molecules found in many bacterial cells |
| 4. | they carry genes vital for normal survival and reproduction |
Subtopic: Tools: Vectors: I |
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In a plasmid such as pBR322:
| I: | Ori controls the copy number of DNA per cell |
| II: | Multiple cloning sites are recognition sequences of common restriction enzymes |
| III: | Genes for antibiotic resistance are used as selectable markers |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II, and III are correct |
Subtopic: Cloning Vector |
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Identify the incorrect statement:
| 1. | The process of separation and purification of expressed protein before marketing is called as downstream processing |
| 2. | DNA precipitation out of a mixture of biomolecules can be achieved by treatment with a divalent cation such as calcium ion |
| 3. | Stirred-tank bioreactors have been designed for availability of oxygen throughout the process |
| 4. | Reporter genes can be used as markers for selecting successfully transformed cells |
Subtopic: Process of Biotech |
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| Assertion (A): | When constructing rDNA, the vector DNA should never be cut by the same restriction endonuclease that was used to cut the donor DNA |
| Reason (R): | Use of same restriction endonuclease will generate same kind of sticky ends retarding the function of DNA ligase. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A) |
| 2. | Both (A) and (R) are True but the (R) is not the correct explanation of the (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Restriction Enzymes - Main Enzymes |
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