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Q. No.Consider the following two statements:
I:
Plasmid can be used as a vector in recombinant DNA technology experiments.
II:
Plasmid is an extrachromosomal, circular, double-stranded DNA found in some bacteria.
1.
Both I and II are correct and II explains I
2.
Both I and II are correct but II does not explain I
3.
I is correct but II is incorrect
4.
Both I and II are incorrect
Subtopic: Tools: Vectors: I | Cloning Vector |
53%
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| Assertion (A): | Thermostable DNA dependent DNA polymerase is used in Polymerase Chain Reaction |
| Reason (R): | Such enzymes do not require primers and can initiate DNA synthesis |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A) |
| 2. | Both (A) and (R) are True but the (R) is not the correct explanation of the (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Polymerase Chain Reaction: PCR |
72%
From NCERT
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| Assertion (A): | In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. |
| Reason (R): | Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A) |
| 2. | Both (A) and (R) are True but the (R) is not the correct explanation of the (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Restriction Enzymes - Main Enzymes |
88%
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| Assertion (A): | When constructing rDNA, the vector DNA should never be cut by the same restriction endonuclease that was used to cut the donor DNA |
| Reason (R): | Use of same restriction endonuclease will generate same kind of sticky ends retarding the function of DNA ligase. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A) |
| 2. | Both (A) and (R) are True but the (R) is not the correct explanation of the (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Restriction Enzymes - Main Enzymes |
76%
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What is true regarding Downstream Processing?
I. This stage is after the biosynthetic stage.
II. The processes include separation and purification.
I. This stage is after the biosynthetic stage.
II. The processes include separation and purification.
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
Subtopic: Large Scale Production |
89%
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In 1970, Hamilton O. Smith, Thomas Kelly and Kent Wilcox isolated and characterized the first type II restriction enzyme that was:
| 1. | EcoR I | 2. | Hind II |
| 3. | Bam HI | 4. | Sma I |
Subtopic: Restriction Enzymes - Main Enzymes |
89%
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In a continuous culture system:
| I: | The used medium is drained out from one side while the fresh medium is added from the other. |
| II: | The cells are maintained in their physiologically most active log/exponential phase. |
| III: | A larger biomass is produced leading to higher yields of desired proteins. |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II and III are correct |
Subtopic: Large Scale Production |
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A plasmid contains two genes for resistance to separate antibiotics. One of the two is insertionally inactivated due to insertion of alien DNA.
| I: | The gene that was not inactivated helps in selecting the transformants. |
| II: | The gene that was insertionally inactivated helps in selection of recombinants. |
| 1. | Only I is correct |
| 2. | Only II is correct |
| 3. | Both I and II are correct |
| 4. | I, II and III are correct |
Subtopic: Cloning Vector | Selection of Recombinant Transformants |
66%
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The polymerase chain reaction:
| I: | is used to synthesize multiple copies of the gene [or DNA] in vitro. |
| II: | uses a thermostable DNA dependent DNA polymerase. |
| III: | is now routinely used to detect HIV in suspected AIDS patient. |
| IV: | is being used to detect mutations in genes in suspected cancer patients. |
| 1. | All except I are true | 2. | Only II and III are true |
| 3. | Only IV is not true | 4. | I, II, III and IV are true |
Subtopic: Polymerase Chain Reaction: PCR |
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Animal cells cannot be transformed with the help of:
| 1. | Microinjection | 2. | Ti Plasmid |
| 3. | Retroviral vectors | 4. | Liposomes |
Subtopic: Transforming Plant & Animal Cell |
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